Anticorps Monoclonal anti-MYL4

• Phare
• Validé par KD/KO

MYL4 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

2F5C1

N° de cat : 67533-1-PBS

Synonymes

2F5C1, MLC1, MYL 4, Myosin light chain 4, Myosin light chain alkali GT-1 isoform

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Galerie de données de validation

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  WB analysis using 67533-1-Ig (same clone as 67533-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67533-1-Ig (MYL4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67533-1-PBS in a different storage buffer formulation.

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  WB analysis using 67533-1-Ig (same clone as 67533-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67533-1-Ig (MYL4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67533-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 67533-1-Ig (same clone as 67533-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67533-1-Ig (MYL4 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67533-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 67533-1-Ig (same clone as 67533-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using MYL4 antibody (67533-1-Ig, Clone: 2F5C1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67533-1-PBS in a different storage buffer formulation.

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  FC experiment of C2C12 using 67533-1-Ig (same clone as 67533-1-PBS)

  1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human MYL4 (67533-1-Ig, Clone:2F5C1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67533-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67533-1-PBS cible MYL4 dans les applications de WB, IHC, IF-P, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MYL4 Protéine recombinante Ag19244
• Nom complet: myosin, light chain 4, alkali; atrial, embryonic
• Masse moléculaire calculée: 197 aa, 22 kDa
• Poids moléculaire observé: 22-25 kDa
• Numéro d’acquisition GenBank: BC030228
• Symbole du gène: MYL4
• Identification du gène (NCBI): 4635
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Myosin light‐chain 4 (MYL4) encodes an essential myosin light chain that is expressed in embryonic whole heart and skeletal muscle. It is specifically expressed in human atria after birth. MYL4 is essential for atrial electrical, functional, and structural integrity, and MYL4 dysfunction can produce severe inherited atrial cardiomyopathy.

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