Anticorps Polyclonal de lapin anti-MYL9

• Phare
• Validé par KD/KO

MYL9 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

8

Avis (1)

N° de cat : 15354-1-AP

Synonymes

20 kDa myosin light chain, LC20, MLC 2C, MLC2, MRLC1, MYL9, Myosin RLC, MYRL2

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Galerie de données de validation

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  WB analysis using 15354-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15354-1-AP (MYL9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Caco-2 using 15354-1-AP

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 15354-1-AP (MYL9 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 15354-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15354-1-AP (MYL9 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15354-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15354-1-AP (MYL9 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 15354-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15354-1-AP (MYL9 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15354-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15354-1-AP (MYL9 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15354-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15354-1-AP (MYL9 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 15354-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using MYL9 antibody (15354-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MCF-7 using 15354-1-AP

  Immunofluorescent analysis of MCF-7 cells, using MYL9 antibody 15354-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of C2C12 using 15354-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using MYL9 antibody (15354-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu de côlon de souris, cellules Caco-2, tissu de côlon de rat
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7, cellules C2C12

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:5000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

15354-1-AP cible MYL9 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MYL9 Protéine recombinante Ag7600
• Nom complet: myosin, light chain 9, regulatory
• Masse moléculaire calculée: 20 kDa
• Poids moléculaire observé: 20 kDa
• Numéro d’acquisition GenBank: BC002648
• Symbole du gène: MYL9
• Identification du gène (NCBI): 10398
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Myosin regulatory light polypeptide 9 (MYL9), also known as MLC2, belongs to the myosin regulatory subunits. It plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation at Thr19 and Ser20. Implicated in cytokinesis, receptor capping, and cell locomotion (PMID:11942626, PMID:2526655). Some studies have demonstrated that MYL9 may play important roles in various human cancers. The expression and phosphorylation of MYL9 (Thr19/Ser20) may be increased in human breast (PMID: 22144583) and liver cancers (PMID: 18648664), while decreased in human colon (PMID: 22752057) and bladder cancers (PMID: 21139803). MYL9 was the only gene differentially expressed in the aged versus young injured arteries in the rat smooth muscle cell layers (PMID:22003410).

Publications

Species	Application	Title
		J Biol Chem; Brain-Derived Neurotrophic Factor regulates LYN kinase mediated Myosin Light Chain Kinase activation to modulate non-muscle myosin II activity in hippocampal neurons.; Authors - Xiao-Bing Li
mouse	IHC	Sci Rep; Proteomic profiling of concurrently isolated primary microvascular endothelial cells, pericytes, and vascular smooth muscle cells from adult mouse heart.; Authors - Zhiping Cao
human	WB	Front Med (Lausanne); Depiction of Aging-Based Molecular Phenotypes With Diverse Clinical Prognosis and Immunological Features in Gastric Cancer.; Authors - Fang He ; KD Validated
human	WB	Oncol Rep; Fenretinide inhibits the proliferation and migration of human liver cancer HepG2 cells by downregulating the activation of myosin light chain kinase through the p38‑MAPK signaling pathway.; Authors - Ling Zhang
mouse	IF	JCI Insight; Transient expansion and myofibroblast conversion of adipogenic lineage precursors mediate bone marrow repair after radiation.; Authors - Leilei Zhong
human	IF	Front Cell Dev Biol; Paracrine HB-EGF signaling reduce enhanced contractile and energetic state of activated decidual fibroblasts by rebalancing SRF-MRTF-TCF transcriptional axis; Authors - Junaid Afzal

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Irem (Verified Customer) (05-18-2022)

20 kDa band can be observed but there are other unspesific bands. Marker: Pageruler protein prestained marker

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Jurkat cells

MYL9 Antibody Western Blot validation (1:1000 dilution) in Jurkat cells (Cat no:15354-1-AP)