Anticorps Polyclonal de lapin anti-MYLK

MYLK Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF/ICC, IF-P, CoIP, ELISA

Conjugaison

Non conjugué

Publications(37)

N° de cat : 21642-1-AP

Synonymes

MLCK108, MLCK1, MLCK, KRP, Kinase-related protein

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Galerie de données de validation

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  WB analysis of mouse uterus using 21642-1-AP

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 21642-1-AP (MYLK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse large intestine using 21642-1-AP

  mouse large intestine tissue were subjected to SDS PAGE followed by western blot with 21642-1-AP (MYLK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse uterus using 21642-1-AP

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 21642-1-AP (MYLK antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 21642-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21642-1-AP (MYLK antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 21642-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21642-1-AP (MYLK antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse ovary using 21642-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse ovary tissue using MYLK antibody (21642-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of human rectal cancer using 21642-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human rectal cancer tissue using MYLK antibody (21642-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 21642-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MYLK antibody (21642-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Applications testées

• Résultats positifs en WB: tissu utérin de souris, tissu de gros intestin de souris
• Résultats positifs en IHC: tissu de côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu ovarien de souris, human rectal cancer tissue
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 30 publications below
• IHC: See 6 publications below
• IF: See 6 publications below
• CoIP: See 1 publications below

Informations sur le produit

21642-1-AP cible MYLK dans les applications de WB, IHC, IF/ICC, IF-P, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MYLK Protéine recombinante Ag16306
• Nom complet: myosin light chain kinase
• Masse moléculaire calculée: 1914 aa, 211 kDa
• Poids moléculaire observé: 135 kDa
• Numéro d’acquisition GenBank: BC113456
• Symbole du gène: MYLK
• Identification du gène (NCBI): 4638
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MYLK(Myosin light chain kinase) is also named as MLCK, MLCK1, MYLK1 and belongs to the protein kinase superfamily. It is a key enzyme in muscle contraction. Its activation is sufficient to enhance paracellular permeability and is required for tight junction barrier regulation in response to Na+-nutrient cotransport, inflammatory cytokines, or pathogenic bacteria(PMID:20404178). The expression of smooth muscle MYLK is tissue-specific. Smooth muscle MYLK generates three different proteins, long MYLK, short MYLK, and telokin. The 130-kDa smooth muscle, or short, MYLK (130 kDa) is expressed in smooth muscles. It has 8 isoforms produced by alternative splicing and alternative initiation and our anti-MYLK antibody can recognize these isforms.

Publications

Species	Application	Title
human	IHC	Adv Sci (Weinh); Elevated SPARC Disrupts the Intestinal Barrier Integrity in Crohn's Disease by Interacting with OTUD4 and Activating the MYD88/NF-κB Pathway; Authors - Jiayu Wang
human	IF	Research (Wash D C); The Mechanics of Tumor Cells Dictate Malignancy via Cytoskeleton-Mediated APC/Wnt/β-Catenin Signaling; Authors - Xi Chen
human	WB	Mucosal Immunol; Transmigrated neutrophils in the intestinal lumen engage ICAM-1 to regulate the epithelial barrier and neutrophil recruitment.; Authors - R Sumagin
pig	WB	Int J Biol Macromol; Proline rich-39 (PR-39) antimicrobial protein alleviated lipopolysaccharide-induced intestinal barrier dysfunction in piglets by altering intestinal flora associated bile acid metabolism and in turn regulating TGR-5/NF-κB/MLCK/MLC pathway; Authors - Zhineng Liu
mouse	WB,IHC	Int J Biol Sci; Aryl Hydrocarbon Receptor Activation Modulates Intestinal Epithelial Barrier Function by Maintaining Tight Junction Integrity.; Authors - Min Yu
rat	IF	FEBS J; Dihydroartemisinin counteracts fibrotic portal hypertension via farnesoid X receptor-dependent inhibition of hepatic stellate cell contraction.; Authors - Wenxuan Xu

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