Anticorps Polyclonal de lapin anti-MYLK2

MYLK2 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 21173-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 21173-1-AP (same clone as 21173-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21173-1-AP (MYLK2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21173-1-PBS in a different storage buffer formulation.

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  WB analysis using 21173-1-AP (same clone as 21173-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 21173-1-AP (MYLK2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21173-1-PBS in a different storage buffer formulation.

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  WB analysis of human skeletal muscle using 21173-1-AP (same clone as 21173-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21173-1-AP (MYLK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21173-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 21173-1-AP (same clone as 21173-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21173-1-AP (MYLK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21173-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 21173-1-AP (same clone as 21173-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 21173-1-AP (MYLK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21173-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 21173-1-AP (same clone as 21173-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 21173-1-AP (MYLK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21173-1-PBS in a different storage buffer formulation.

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Informations sur le produit

21173-1-PBS cible MYLK2 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MYLK2 Protéine recombinante Ag15424
• Nom complet: myosin light chain kinase 2
• Masse moléculaire calculée: 596 aa, 65 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: BC069627
• Symbole du gène: MYLK2
• Identification du gène (NCBI): 85366
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MYLK2, also named as MLCK2, belongs to the protein kinase superfamily and CAMK Ser/Thr protein kinase family. MYLK2 catalyze the reaction: ATP + [myosin light-chain] = ADP + [myosin light-chain] phosphate. MYLK2 is implicated in the level of global muscle contraction and cardiac function. MYLK2 phosphorylates a specific serine in the N-terminus of a myosin light chain. Mutation of MYLK2 will cause the cardiomyopathy familial hypertrophic (CMH).

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