Anticorps Polyclonal de lapin anti-MYLK4

• Phare
• Validé par KD/KO

MYLK4 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 24309-1-AP

Synonymes

MLCK4, MYLK4, SgK085, Sugen kinase 85

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Galerie de données de validation

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  WB analysis using 24309-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24309-1-AP (MYLK4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 24309-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24309-1-AP (MYLK4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 24309-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 24309-1-AP (MYLK4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 24309-1-AP

  IP result of anti-MYLK4 (IP:24309-1-AP, 4ug; Detection:24309-1-AP 1:500) with HEK-293 cells lysate 3600ug.

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  IHC staining of human heart using 24309-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24309-1-AP (MYLK4 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 24309-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24309-1-AP (MYLK4 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human tonsillitis using 24309-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24309-1-AP (MYLK4 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human tonsillitis using 24309-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24309-1-AP (MYLK4 Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules L02
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu cardiaque humain, tissu d'amygdalite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

24309-1-AP cible MYLK4 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MYLK4 Protéine recombinante Ag19314
• Nom complet: myosin light chain kinase family, member 4
• Masse moléculaire calculée: 388 aa, 45 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC132831
• Symbole du gène: MYLK4
• Identification du gène (NCBI): 340156
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MYLK4(Myosin light chain kinase family member 4) is also named as SGK085 and belongs to the CAMK Ser/Thr protein kinase family. MLCKs are activated by Ca2+-calmodulin complexes and require Mg2+ or Ca2+ as a co-factor. It has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
	WB,IHC	Proc Natl Acad Sci U S A; Cardiac myosin light chain is phosphorylated by Ca2+/calmodulin-dependent and -independent kinase activities.; Authors - Audrey N Chang
human	WB	J Exp Clin Cancer Res; MYLK4 promotes tumor progression through the activation of epidermal growth factor receptor signaling in osteosarcoma.; Authors - Mengkai Yang ; KD Validated
	IF	bioRxiv; GATA1-defective immune-megakaryocytes as possible drivers of idiopathic pulmonary fibrosis; Authors - Francesca Gobbo

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