Anticorps Polyclonal de lapin anti-MYO18A

• Phare
• Validé par KD/KO

MYO18A Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14611-1-PBS

Synonymes

CD245, KIAA0216, Molecule associated with JAK3 N-terminus, myosin XVIIIA, myosinXVIIIA

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Galerie de données de validation

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  WB analysis of Jurkat using 14611-1-AP (same clone as 14611-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14611-1-AP (MYO18A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14611-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 14611-1-AP (same clone as 14611-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14611-1-AP (MYO18A Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14611-1-PBS in a different storage buffer formulation.

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  WB analysis of Neuro-2a using 14611-1-AP (same clone as 14611-1-PBS)

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 14611-1-AP (MYO18A Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14611-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 14611-1-AP (same clone as 14611-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 14611-1-AP (MYO18A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14611-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 14611-1-AP (same clone as 14611-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14611-1-AP (MYO18A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14611-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 14611-1-AP (same clone as 14611-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14611-1-AP (MYO18A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14611-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 14611-1-AP (same clone as 14611-1-PBS)

  IP result of anti-MYO18A (IP:14611-1-AP, 3ug; Detection:14611-1-AP 1:300) with HeLa cells lysate 3000ug. This data was developed using the same antibody clone with 14611-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 14611-1-AP (same clone as 14611-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 14611-1-AP (MYO18A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14611-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 14611-1-AP (same clone as 14611-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 14611-1-AP (MYO18A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14611-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 14611-1-AP (same clone as 14611-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14611-1-AP (MYO18A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14611-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14611-1-PBS cible MYO18A dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MYO18A Protéine recombinante Ag6185
• Nom complet: myosin XVIIIA
• Masse moléculaire calculée: 233 kDa
• Poids moléculaire observé: 230 kDa, 190 kDa
• Numéro d’acquisition GenBank: BC039612
• Symbole du gène: MYO18A
• Identification du gène (NCBI): 399687
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MYO18A is an unconventional myosin that may play a role in the stabilization and organization of the actin cytoskeleton. Recently MYO18A has been identified as the receptor of SP and is responsible for clearance of SP-A-opsonized bacteria in in vivo infection. Two different isoforms of mouse Myo18A have been identified: Myo18A-alpha and Myo18A-beta as 230- and 190-kDa proteins, respectively. Myo18a-alpha colocalizes with the ER-Golgi complex and with membrane ruffles and filopodia, while Myo18a-beta localizes diffusely to the cytoplasm.

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