Anticorps Polyclonal de lapin anti-MYO1G

• Phare
• Validé par KD/KO

MYO1G Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 25772-1-PBS

Synonymes

HA 2, Minor histocompatibility antigen HA-2, myosin IG, Unconventional myosin-Ig

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Galerie de données de validation

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  WB analysis of Jurkat using 25772-1-AP (same clone as 25772-1-PBS)

  WB result of MYO1G antibody (25772-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MYO1G transfected Jurkat cells. This data was developed using the same antibody clone with 25772-1-PBS in a different storage buffer formulation.

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  WB analysis using 25772-1-AP (same clone as 25772-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 25772-1-AP (MYO1G antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25772-1-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 25772-1-AP (same clone as 25772-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using MYO1G antibody (25772-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25772-1-PBS in a different storage buffer formulation.

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Informations sur le produit

25772-1-PBS cible MYO1G dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MYO1G Protéine recombinante Ag22696
• Nom complet: myosin IG
• Masse moléculaire calculée: 1018 aa, 116 kDa
• Poids moléculaire observé: 110-120 kDa
• Numéro d’acquisition GenBank: BC113544
• Symbole du gène: MYO1G
• Identification du gène (NCBI): 64005
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MYO1G is a plasma membrane-associated class I myosin that is abundant in T and B lymphocytes and mast cells and expressed specifically in the haematopoietic system. Class I myosins have been implicated in various cellular processes relying on actin-dependent membrane dynamics such as endocytosis, secretion, adhesion, motility and regulation of mechanosensitive channels (PMID: 19968988). MYO1G localizes to the plasma membrane linked to PIP2 and PIP3, is particularly enriched at cell-surface microvilli, and associates in an ATP-releasable manner to the actin cytoskeleton (PMID: 24310084). MYO1G can be detected as about 110-120 kDa.

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