Anticorps Polyclonal de lapin anti-MYO5B

MYO5B Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 29996-1-PBS

Synonymes

---

Galerie de données de validation

• 

  WB analysis using 29996-1-AP (same clone as 29996-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29996-1-AP (MYO5B antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29996-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 29996-1-AP (same clone as 29996-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29996-1-AP (MYO5B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29996-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 29996-1-AP (same clone as 29996-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29996-1-AP (MYO5B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29996-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 29996-1-AP (same clone as 29996-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 29996-1-AP (MYO5B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29996-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

29996-1-PBS cible MYO5B dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MYO5B Protéine recombinante Ag31590
• Nom complet: myosin VB
• Masse moléculaire calculée: 214KD
• Poids moléculaire observé: 200 kDa
• Numéro d’acquisition GenBank: NM_001080467
• Symbole du gène: MYO5B
• Identification du gène (NCBI): 4645
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MYO5B is a member of the class V of unconventional, dimeric nonfilamentous myosins, which may be involved in vesicular trafficking. Members of the Rab family of small GTPases, consisting of Rab11a, Rab11b, and Rab25, have recently emerged as potential mediators of vesicle transport by MYO5B (PMID: 21282656). MYO5B is required for polarization of hepatocytes and mutations of MYO5B could disrupt epithelial cell polarity. Loss-of-Function of MYO5B caused by mutation is the main cause of microvillus inclusion disease (MVID). Recently it was reported that the expression of MYO5B was downregulated in gastric cancer and inactivation of MYO5B may contribute to tumorigenesis (PMID: 22134786).

{{ptg:RelatedPrimaryAntibodies}}