Anticorps Polyclonal de lapin anti-MYO7A

MYO7A Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 20720-1-PBS

Synonymes

DFNA11, DFNB2, myosin VIIA, MYOVIIA, MYU7A

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Galerie de données de validation

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  WB analysis of L02 using 20720-1-AP (same clone as 20720-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 20720-1-AP (MYO7A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20720-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 20720-1-AP (same clone as 20720-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 20720-1-AP (MYO7A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20720-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 20720-1-AP (same clone as 20720-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20720-1-AP (MYO7A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20720-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 20720-1-AP (same clone as 20720-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20720-1-AP (MYO7A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20720-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 20720-1-AP (same clone as 20720-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20720-1-AP (MYO7A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20720-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 20720-1-AP (same clone as 20720-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20720-1-AP (MYO7A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20720-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 20720-1-AP (same clone as 20720-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MYO7A antibody (20720-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 20720-1-PBS in a different storage buffer formulation.

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Informations sur le produit

20720-1-PBS cible MYO7A dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: myosin VIIA
• Masse moléculaire calculée: 254 kDa
• Poids moléculaire observé: 240-250 kDa
• Numéro d’acquisition GenBank: NM_000260
• Symbole du gène: MYO7A
• Identification du gène (NCBI): 4647
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MYO7A, also named a USH1B, is one of myosins protein which are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments. In retina, MYO7A might play a role in trafficking of ribbon-synaptic vesicle complexes and renewal of the outer photoreceptors disks. In inner ear, it might maintain the rigidity of stereocilia during the dynamic movements of the bundle. It is involved in hair-cell vesicle trafficking of aminoglycosides, which are known to induce ototoxicity. Defects in MYO7A are the cause of Usher syndrome type 1B (USH1B). Defects in MYO7A are the cause of deafness autosomal recessive type 2 (DFNB2). Defects in MYO7A are the cause of deafness autosomal dominant type 11 (DFNA11). The antibody is specific to MYO7A.

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