Anticorps Polyclonal de lapin anti-MYO9B

• Phare
• Validé par KD/KO

MYO9B Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(12)

Avis (1)

N° de cat : 12432-1-AP

Synonymes

Unconventional myosin-IXb, Unconventional myosin-9b, MYR5, myosin IXB, MYO9B variant protein

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Galerie de données de validation

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  WB analysis of mouse thymus using 12432-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12432-1-AP (MYO9B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 12432-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12432-1-AP (MYO9B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 12432-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 12432-1-AP (MYO9B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 12432-1-AP

  IP result of anti-MYO9B (IP:12432-1-AP, 5ug; Detection:12432-1-AP 1:500) with HepG2 cells lysate 2800ug.

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  IHC staining of human tonsillitis using 12432-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12432-1-AP (MYO9B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu de thymus de souris, cellules HL-60, cellules Jurkat
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu d'amygdalite humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 7 publications below
• WB: See 10 publications below
• IF: See 7 publications below

Informations sur le produit

12432-1-AP cible MYO9B dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MYO9B Protéine recombinante Ag3108
• Nom complet: myosin IXB
• Masse moléculaire calculée: 2023 aa, 223 kDa
• Poids moléculaire observé: 230-250 kDa
• Numéro d’acquisition GenBank: BC018108
• Symbole du gène: MYO9B
• Identification du gène (NCBI): 4650
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Nat Commun; Myo9b mutations are associated with altered dendritic cell functions and increased susceptibility to autoimmune diabetes onset; Authors - Jing Zhang
human,mouse	WB,IF	Nat Commun; An allosteric inhibitor of RhoGAP class-IX myosins suppresses the metastatic features of cancer cells; Authors - Despoina Kyriazi ; KD Validated
human	WB	J Thromb Haemost; The RhoA regulators Myo9b and GEF-H1 are targets of cyclic nucleotide-dependent kinases in platelets.; Authors - Shane Comer
human	WB,IF	Cell Rep; Specific Myosins Control Actin Organization, Cell Morphology, and Migration in Prostate Cancer Cells.; Authors - Katarzyna A Makowska ; KD Validated
mouse	IF	J Bone Miner Res; The RhoGAP Myo9b Promotes Bone Growth by Mediating Osteoblastic Responsiveness to IGF-1.; Authors - Brooke K McMichael ; KO Validated
mouse	WB	J Immunol; TSAd Plays a Major Role in Myo9b-Mediated Suppression of Malignant Pleural Effusion by Regulating TH1/TH17 Cell Response.; Authors - Feng-Shuang Yi

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sammy (Verified Customer) (11-20-2023)

Lots of speckles, but also observed at focal adhesions where it is reported in the literature.

• Applications: Immunofluorescence, Flow Cytometry
• Primary Antibody Dilution: 1 in 200
• Cell Tissue Type: HUVEC

MYO9B Antibody Immunofluorescence,Flow Cytometry validation (1 in 200 dilution) in HUVEC (Cat no:12432-1-AP)

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