Anticorps Polyclonal de lapin anti-MYOD1

MYOD1 Polyclonal Antibody for FC, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (7)

Applications

WB, IHC, IF, FC, ChIP, ELISA

Conjugaison

Non conjugué

Publications

83

Avis (1)

N° de cat : 18943-1-AP

Synonymes

bHLHc1, Myf 3, MYF3, MYOD, MYOD1, myogenic differentiation 1, Myogenic factor 3, PUM

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Galerie de données de validation

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  WB analysis using 18943-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 18943-1-AP (MYOD1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18943-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18943-1-AP (MYOD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 18943-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 18943-1-AP (MYOD1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 18943-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 18943-1-AP (MYOD1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 18943-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 18943-1-AP (MYOD1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 18943-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 18943-1-AP (MYOD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse embryo using 18943-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 18943-1-AP (MYOD1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse embryo using 18943-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 18943-1-AP (MYOD1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse embryo using 18943-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 18943-1-AP (MYOD1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of C2C12 using 18943-1-AP

  1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human MYOD1 (18943-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, tissu cardiaque de rat, tissu cardiaque humain, tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu embryonnaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules C2C12,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 64 publications below
• IHC: See 10 publications below
• IF: See 21 publications below
• ChIP: See 1 publications below

Informations sur le produit

18943-1-AP cible MYOD1 dans les applications de WB, IHC, IF, FC, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Chèvre, Humain, Lapin, poisson-zèbre, porc, poulet, souris, duck
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MYOD1 Protéine recombinante Ag13512
• Nom complet: myogenic differentiation 1
• Masse moléculaire calculée: 320 aa, 35 kDa
• Poids moléculaire observé: 35-45 kDa
• Numéro d’acquisition GenBank: BC064493
• Symbole du gène: MYOD1
• Identification du gène (NCBI): 4654
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MYOD1, also named as BHLHC1 or MYF3, is a 320 amino acid protein, which promotes the transcriptional activity of MYOD1 through its CDK9-mediated phosphorylation. This phosphorylation promotes its function in muscle differentiation. MYOD1 acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. MYOD1 together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. he calcualted molecular weight of MYOD1 is 34 kDa, but modified MYOD1 is about 45 kDa. (PMID: 12037670)

Publications

Species	Application	Title
human	IF	Nature; A plant-derived natural photosynthetic system for improving cell anabolism; Authors - Pengfei Chen
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
	WB	Nature; SAR1B senses leucine levels to regulate mTORC1 signalling.; Authors - Jie Chen
mouse	WB	Nat Commun; The transcription factor NF-Y participates to stem cell fate decision and regeneration in adult skeletal muscle.; Authors - Giovanna Rigillo
mouse	WB	J Cachexia Sarcopenia Muscle; circRNAome profiling reveals circFgfr2 regulates myogenesis and muscle regeneration via a feedback loop.; Authors - Junyu Yan
human	WB,IHC	Cancer Res; YAP1-mediated suppression of USP31 enhances NF-κB activity to promote sarcomagenesis.; Authors - Shuai Ye

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Cassandra (Verified Customer) (02-04-2021)

This antibody worked quite well in my IF staining. I used 1 to 100 antibody dilution.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Satellite cell

MYOD1 Antibody Immunofluorescence validation (1:100 dilution) in Satellite cell (Cat no:18943-1-AP)

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