Anticorps Polyclonal de lapin anti-MYOD1

• Phare
• Validé par KD/KO

MYOD1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 18943-1-PBS

Synonymes

bHLHc1, Class C basic helix-loop-helix protein 1, Myf 3, MYF3, Myf-3

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Galerie de données de validation

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  WB analysis using 18943-1-AP (same clone as 18943-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 18943-1-AP (MYOD1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18943-1-PBS in a different storage buffer formulation.

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  WB analysis using 18943-1-AP (same clone as 18943-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18943-1-AP (MYOD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18943-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 18943-1-AP (same clone as 18943-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 18943-1-AP (MYOD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18943-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse skeletal muscle using 18943-1-AP (same clone as 18943-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 18943-1-AP (MYOD1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18943-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat heart using 18943-1-AP (same clone as 18943-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 18943-1-AP (MYOD1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18943-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 18943-1-AP (same clone as 18943-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 18943-1-AP (MYOD1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18943-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse embryo using 18943-1-AP (same clone as 18943-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 18943-1-AP (MYOD1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18943-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse embryo using 18943-1-AP (same clone as 18943-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 18943-1-AP (MYOD1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18943-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse embryo using 18943-1-AP (same clone as 18943-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 18943-1-AP (MYOD1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18943-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 18943-1-AP (same clone as 18943-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MYOD1 antibody (18943-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 18943-1-PBS in a different storage buffer formulation.

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Informations sur le produit

18943-1-PBS cible MYOD1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MYOD1 Protéine recombinante Ag13512
• Nom complet: myogenic differentiation 1
• Masse moléculaire calculée: 320 aa, 35 kDa
• Poids moléculaire observé: 35-45 kDa
• Numéro d’acquisition GenBank: BC064493
• Symbole du gène: MYOD1
• Identification du gène (NCBI): 4654
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MYOD1, also named as BHLHC1 or MYF3, is a 320 amino acid protein, which promotes the transcriptional activity of MYOD1 through its CDK9-mediated phosphorylation. This phosphorylation promotes its function in muscle differentiation. MYOD1 acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. MYOD1 together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. he calcualted molecular weight of MYOD1 is 34 kDa, but modified MYOD1 is about 45 kDa. (PMID: 12037670)

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