Anticorps Polyclonal de lapin anti-MYOF

MYOF Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 19548-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 19548-1-AP (same clone as 19548-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 19548-1-AP (MYOF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19548-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 19548-1-AP (same clone as 19548-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 19548-1-AP (Myoferlin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19548-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 19548-1-AP (same clone as 19548-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 19548-1-AP (Myoferlin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19548-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 19548-1-AP (same clone as 19548-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19548-1-AP (Myoferlin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19548-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 19548-1-AP (same clone as 19548-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19548-1-AP (Myoferlin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19548-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 19548-1-AP (same clone as 19548-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Myoferlin antibody (19548-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 19548-1-PBS in a different storage buffer formulation.

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Informations sur le produit

19548-1-PBS cible MYOF dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: myoferlin
• Masse moléculaire calculée: 235 kDa
• Poids moléculaire observé: 235-250 kDa
• Numéro d’acquisition GenBank: NM_133337
• Symbole du gène: MYOF
• Identification du gène (NCBI): 26509
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MYOF, also named as FER1L3 and KIAA1207, belongs to the ferlin family. MYOF is a calcium/phospholipid-binding protein that plays a role in the plasmalemma repair mechanism of endothelial cells that permits rapid resealing of membranes disrupted by mechanical stress. It is involved in endocytic recycling. MYOF is implicated in VEGF signal transduction by regulating the levels of the receptor KDR. The antibody can recognize all the 8 isoforms of MYOF.

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