Anticorps Polyclonal de lapin anti-MYOM3

MYOM3 Polyclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 17692-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 17692-1-AP (same clone as 17692-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17692-1-AP (MYOM3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17692-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse skeletal muscle using 17692-1-AP (same clone as 17692-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17692-1-AP (MYOM3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17692-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse skeletal muscle using 17692-1-AP (same clone as 17692-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17692-1-AP (MYOM3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17692-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse skeletal muscle using 17692-1-AP (same clone as 17692-1-PBS)

  IP result of anti-MYOM3 (IP:17692-1-AP, 4ug; Detection:17692-1-AP 1:1000) with mouse skeletal muscle tissue lysate 1280 ug. This data was developed using the same antibody clone with 17692-1-PBS in a different storage buffer formulation.

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  IHC staining of human skeletal muscle using 17692-1-AP (same clone as 17692-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17692-1-AP (MYOM3 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 17692-1-PBS in a different storage buffer formulation.

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  IHC staining of human skeletal muscle using 17692-1-AP (same clone as 17692-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17692-1-AP (MYOM3 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 17692-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 17692-1-AP (same clone as 17692-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 17692-1-AP (MYOM3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17692-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 17692-1-AP (same clone as 17692-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 17692-1-AP (MYOM3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17692-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse skeletal muscle using 17692-1-AP (same clone as 17692-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using MYOM3 antibody (17692-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17692-1-PBS in a different storage buffer formulation.

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Informations sur le produit

17692-1-PBS cible MYOM3 dans les applications de WB, IHC, IF-P, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MYOM3 Protéine recombinante Ag11849
• Nom complet: myomesin family, member 3
• Masse moléculaire calculée: 162 kDa, 136 kDa
• Poids moléculaire observé: 162 kDa
• Numéro d’acquisition GenBank: BC067101
• Symbole du gène: MYOM3
• Identification du gène (NCBI): 127294
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MYOM3 (myomesin 3) is a structural component of the M-band in striated muscle and is involved in sarcomere stability and resistance during intense or sustained stretching. MYOM3 can be detected mainly in intermediate-speed fibers of skeletal muscle. Recently high levels of MYOM3 fragments were detected in sera from patients with muscular dystrophy, including Duchenne muscular dystrophy (DMD). MYOM3 fragments may be used as serum biomarkers for DMD and other neuromuscular disorders. This antibody recognizes the intact MYOM3 protein (160-162 kDa) as well as MYOM3 fragments (100 kDa and 130 kDa). (26060189)

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