Anticorps Recombinant de lapin anti-MYOM3

MYOM3 Recombinant Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

240981F9

N° de cat : 83852-3-PBS

Synonymes

Myomesin-3, Myomesin family member 3, Myomesin 3, 240981F9

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Galerie de données de validation

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  WB analysis using 83852-3-RR (same clone as 83852-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83852-3-RR (MYOM3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83852-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 83852-3-RR (same clone as 83852-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 83852-3-RR (MYOM3 antibody) at dilution of 1:0 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83852-3-PBS in a different storage buffer formulation.

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  IF Staining of mouse skeletal muscle using 83852-3-RR (same clone as 83852-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using MYOM3 antibody (83852-3-RR, Clone: 240981F9 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83852-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83852-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83852-3-RR against Human MYOM3 were performed. The affinity constant is 0.106 nM.

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Informations sur le produit

83852-3-PBS cible MYOM3 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MYOM3 Protéine recombinante Ag11849
• Nom complet: myomesin family, member 3
• Masse moléculaire calculée: 162 kDa, 136 kDa
• Poids moléculaire observé: 162 kDa
• Numéro d’acquisition GenBank: BC067101
• Symbole du gène: MYOM3
• Identification du gène (NCBI): 127294
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MYOM3 (myomesin 3) is a structural component of the M-band in striated muscle and is involved in sarcomere stability and resistance during intense or sustained stretching. MYOM3 can be detected mainly in intermediate-speed fibers of skeletal muscle. Recently high levels of MYOM3 fragments were detected in sera from patients with muscular dystrophy, including Duchenne muscular dystrophy (DMD). MYOM3 fragments may be used as serum biomarkers for DMD and other neuromuscular disorders.

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