Anticorps Polyclonal de lapin anti-MYOT

MYOT Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10731-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of mouse skeletal muscle using 10731-1-AP (same clone as 10731-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10731-1-AP (MYOT antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10731-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse skeletal muscle using 10731-1-AP (same clone as 10731-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10731-1-AP (MYOT antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10731-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 10731-1-AP (same clone as 10731-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10731-1-AP (MYOT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10731-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 10731-1-AP (same clone as 10731-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 10731-1-AP (MYOT antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10731-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 10731-1-AP (same clone as 10731-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 10731-1-AP (MYOT antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10731-1-PBS in a different storage buffer formulation.

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Informations sur le produit

10731-1-PBS cible MYOT dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MYOT Protéine recombinante Ag1112
• Nom complet: myotilin
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 55-57 kDa, 35 kDa
• Numéro d’acquisition GenBank: BC005376
• Symbole du gène: MYOT
• Identification du gène (NCBI): 9499
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MYOT (myotilin) is a structural protein of the striated muscle Z-discs. It interacts with both actinin and filamin, forming a complex to maintain structural stability of muscles. In adult tissues, myotilin is mainly expressed in skeletal and cardiac muscles and in the peripheral nerves. Missense mutations of myotilin cause limb girdle muscular dystrophy 1A and some other myopathy. Two isoforms of MYOT exist due to the alternative splicing. This antibody can detect both of isoforms around 57 kDa and 35 kDa.

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