Anticorps Polyclonal de lapin anti-MYPN-Specific

MYPN-Specific Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 16180-1-PBS

Synonymes

MYPN, MYOP, Myopalladin

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Galerie de données de validation

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  WB analysis using 16180-1-AP (same clone as 16180-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16180-1-AP (MYPN-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16180-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 16180-1-AP (same clone as 16180-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16180-1-AP (MYPN-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16180-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 16180-1-AP (same clone as 16180-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16180-1-AP (MYPN-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16180-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 16180-1-AP (same clone as 16180-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16180-1-AP (MYPN-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16180-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 16180-1-AP (same clone as 16180-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16180-1-AP (MYPN-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16180-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 16180-1-AP (same clone as 16180-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16180-1-AP (MYPN-Specific antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16180-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 16180-1-AP (same clone as 16180-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 16180-1-AP (MYPN-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16180-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 16180-1-AP (same clone as 16180-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16180-1-AP (MYPN-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16180-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 16180-1-AP (same clone as 16180-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16180-1-AP (MYPN-Specific Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16180-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 16180-1-AP (same clone as 16180-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16180-1-AP (MYPN-Specific Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16180-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 16180-1-AP (same clone as 16180-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16180-1-AP (MYPN-Specific Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16180-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 16180-1-AP (same clone as 16180-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16180-1-AP (MYPN-Specific Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16180-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 16180-1-AP (same clone as 16180-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MYPN-Specific antibody (16180-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 16180-1-PBS in a different storage buffer formulation.

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Informations sur le produit

16180-1-PBS cible MYPN-Specific dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: myopalladin
• Masse moléculaire calculée: 145 kDa
• Poids moléculaire observé: 145 kDa
• Numéro d’acquisition GenBank: NM_032578
• Symbole du gène: MYPN
• Identification du gène (NCBI): 84665
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MYPN is a component of the sarcomere that tethers nebulin in skeletal muscle and nebulette in cardiac muscle to alpha-actinin at the Z lines (from OMIM). Myopalladin and palladin share C-terminal fragment in sequence. While myopalladin are most abundant in skeletal and cardiac muscle, palladin is ubiquitously expressed in the organs of developing vertebrates. This antibody is specific to myopalladin.

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