Anticorps Polyclonal de lapin anti-MYPT1

• Phare
• Validé par KD/KO

MYPT1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

13

Avis (2)

N° de cat : 22117-1-AP

Synonymes

MBS, MYPT1, PPP1R12A

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Galerie de données de validation

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  WB analysis using 22117-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22117-1-AP (MYPT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 22117-1-AP

  WB result of MYPT1 antibody (22117-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MYPT1 transfected HEK-293 cells.

• 

  WB analysis using 22117-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22117-1-AP (MYPT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 22117-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22117-1-AP (MYPT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 22117-1-AP

  IP result of anti-MYPT1 (IP:22117-1-AP, 4ug; Detection:22117-1-AP 1:1000) with HEK-293 cells lysate 4000 ug.

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  IHC staining of human heart using 22117-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22117-1-AP (MYPT1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 22117-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22117-1-AP (MYPT1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human gliomas using 22117-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 22117-1-AP (MYPT1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human gliomas using 22117-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 22117-1-AP (MYPT1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 22117-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 22117-1-AP (MYPT1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 22117-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 22117-1-AP (MYPT1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 22117-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 22117-1-AP (MYPT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules C2C12, cellules C6, cellules HeLa, cellules Jurkat, cellules MCF-7
• Résultats positifs en IP: cellules HEK-293,
• Résultats positifs en IHC: tissu cardiaque humain, tissu de gliome humain, tissu de muscle squelettique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:3000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

22117-1-AP cible MYPT1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MYPT1 Protéine recombinante Ag17496
• Nom complet: protein phosphatase 1, regulatory (inhibitor) subunit 12A
• Masse moléculaire calculée: 1030 aa, 115 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: BC111752
• Symbole du gène: PPP1R12A
• Identification du gène (NCBI): 4659
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Myosin phosphatase target subunit 1(MYPT1), which is also called PPP1R12A, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Phosphorylation of MYPT1 at Thr696 and Thr853 results in phosphatase inhibition and cytoskeletal reorganization. Several transcript variants encoding different isoforms have been found for this gene.

Publications

Species	Application	Title
human	WB	Int J Mol Sci; RhoA/ROCK Pathway Activation is Regulated by AT1 Receptor and Participates in Smooth Muscle Migration and Dedifferentiation via Promoting Actin Cytoskeleton Polymerization.; Authors - Yan Qi
rat	WB	Sci Rep; MicroRNA-223 Attenuates Hypoxia-induced Vascular Remodeling by Targeting RhoB/MLC2 in Pulmonary Arterial Smooth Muscle Cells.; Authors - Yan Zeng
mouse	WB	Sci Rep; Nicotine facilitates VSMC dysfunction through a miR-200b/RhoGDIA/cytoskeleton module.; Authors - Dongli Liang
rat	WB	Front Pharmacol; Madagascine Induces Vasodilatation via Activation of AMPK.; Authors - Dapeng Chen
pig	WB, IF	Cells; Mitotic Acetylation of Microtubules Promotes Centrosomal PLK1 Recruitment and Is Required to Maintain Bipolar Spindle Homeostasis.; Authors - Sylvia Fenosoa Rasamizafy ; KD Validated
human	WB	Food Funct; Chlorogenic acid improves the intestinal barrier by relieving endoplasmic reticulum stress and inhibiting ROCK/MLCK signaling pathways.; Authors - Luqing Song

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Siddharth (Verified Customer) (07-28-2023)

Good for western blotting. Band detected around 120 KDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: M2-10B4 cell-line (mouse bone marrow stromal cells)

FH

Claudia (Verified Customer) (10-19-2021)

Unmasking microwave: potencia medium, ph6, 10 min., cool 35 min., room temperature.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Porcine coronaryarteries on paraffin-embedded sections