Anticorps Recombinant de lapin anti-MYSM1-Specific

MYSM1-Specific Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

243158A9

N° de cat : 85927-1-RR

Synonymes

MYSM1, 2A DUB, 2ADUB, 2A-DUB, Deubiquitinase MYSM1

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Galerie de données de validation

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  WB analysis using 85927-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85927-1-RR (MYSM1-Specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 85927-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85927-1-RR (MYSM1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 85927-1-RR

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 85927-1-RR (MYSM1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules HEK-293, cellules HepG2, cellules NIH/3T3, cellules RAW 264.7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85927-1-RR cible MYSM1-Specific dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: Myb-like, SWIRM and MPN domains 1
• Masse moléculaire calculée: 95 kDa
• Poids moléculaire observé: 81 kDa
• Numéro d’acquisition GenBank: NM_001085487
• Symbole du gène: MYSM1
• Identification du gène (NCBI): 114803
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MYSM1, also named as KIAA1915, belongs to the peptidase M67A family and MYSM1 subfamily. MYSM1 is a metalloprotease that specifically deubiquitinates monoubiquitinated histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A leads to facilitate the phosphorylation and dissociation of histone H1 from the nucleosome. MYSM1 acts as a coactivator by participating in the initiation and elongation steps of androgen receptor (AR)-induced gene activation. It catalyzes the reaction: Ubiquitin C-terminal thioester + H2O = ubiquitin + a thiol. MYSM1 is a marker of cancer. The antibody is specific to MYSM1.

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