Anticorps Recombinant de lapin anti-MCP-1/CCL2

MCP-1/CCL2 Recombinant Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

230483H3

N° de cat : 83157-4-PBS

Synonymes

Ccl2, MCP1, MCP-1, C-C motif chemokine 2, CCL 2

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Galerie de données de validation

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  Sandwich ELISA standard curve of MP00168-4

  Sandwich ELISA standard curve of MP00168-4, Mouse MCP-1 Recombinant Matched Antibody Pair - PBS only. 83157-6-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0427. 83157-4-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

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  Sample test of MP00168-4

  J774A.1 mouse reticulum cell sarcoma macrophage cells (1 x 10*6 cells/mL) were cultured for 3 days in DMEM containing 10% fetal bovine serum. Cells were untreated or treated with 500 ng/mL recombinant mouse IFN-γ and 10 ng/mL LPS. An aliquot of the cell culture supernatants were removed, assayed for mouse MCP-1, and measured 6,956.6 pg/mL and 58,869.9 pg/mL.

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  Sample test of MP00168-4

  Serum of sixteen mice was measured. The MCP-1 concentration of detected samples was determined to be 77.0 pg/mL with a range of 39.7 - 135.3 pg/mL.

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  Affinity and Kinetic Characterization of 83157-4-PBS

  Biolayer interferometry (BLl) kinetic assays of 83157-4-PBS against Mouse MCP-1/CCL2 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

83157-4-PBS cible MCP-1/CCL2 dans les applications de Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MCP-1/CCL2 Protéine recombinante Eg0427
• Nom complet: chemokine (C-C motif) ligand 2
• Numéro d’acquisition GenBank: NM-011333
• Symbole du gène: MCP-1/CCL2
• Identification du gène (NCBI): 20296
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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