Anticorps Recombinant de lapin anti-Midkine

Midkine Recombinant Antibody for IF/ICC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

241880H1

N° de cat : CL488-84815

Synonymes

MDK, 241880H1, Amphiregulin-associated protein, Neurite outgrowth-promoting factor 2, Neurite outgrowth-promoting protein

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Galerie de données de validation

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  IF Staining of HeLa using CL488-84815

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 Midkine antibody (CL488-84815, Clone: 241880H1 ) at dilution of 1:200, CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-84815 cible Midkine dans les applications de IF/ICC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Midkine Protéine recombinante Ag24547
• Nom complet: midkine (neurite growth-promoting factor 2)
• Masse moléculaire calculée: 16 kDa
• Numéro d’acquisition GenBank: BC011704
• Symbole du gène: Midkine
• Identification du gène (NCBI): 4192
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Midkine is a heparin-binding growth factor identified over 20 years ago and enhances the survival, migration and many other activities of target cells. Midkine is rich in both basic amino acids and cysteine, and is not related to most other growth factors/cytokines. It is strongly expressed during embryonic periods, especially at the midgestation stage, and plays important roles in development, especially in neurogenesis. Midkine expression in adult tissue is generally weak or undetectable, and it is induced upon injury and exerts many activities related to tissue repair. The biological activities of midkine in malignant tumors include proliferation, angiogenesis, invasion and metastasis. Various cancers express significantly higher levels of the midkine protein in early stage tumor tissues than in adjacent normal tissue.

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