Anticorps Polyclonal de lapin anti-MLKL

MLKL Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 30145-1-AP

Synonymes

mixed lineage kinase domain like

---

Galerie de données de validation

• 

  WB analysis using 30145-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30145-1-AP (Mlkl antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 30145-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30145-1-AP (Mlkl antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of NIH/3T3 using 30145-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Mlkl antibody (30145-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules NIH/3T3, cellules 4T1, cellules HSC-T6
• Résultats positifs en IF/ICC: cellules NIH/3T3,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

30145-1-AP cible MLKL dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MLKL Protéine recombinante Ag32514
• Nom complet: mixed lineage kinase domain-like
• Masse moléculaire calculée: 54kd
• Poids moléculaire observé: 54 kDa
• Numéro d’acquisition GenBank: NM_001310613
• Symbole du gène: Mlkl
• Identification du gène (NCBI): 74568
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Mixed lineage kinase domain-like pseudokinase (Mlkl) belongs to the protein kinase superfamily and has two MWs of 54 and 30 kDa. Mlkl plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in the necroptosis pathway. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children.

Publications

Species	Application	Title
mouse	WB	Physiol Res; Trimethylamine Induced Chronic Kidney Injury by Activating the ZBP1-NLRP3 Inflammasome Pathway; Authors - L Bai
mouse	WB	FASEB J; Metformin switches cell death modes to soothe the apical periodontitis via ZBP1; Authors - Hui Liu

{{ptg:RelatedPrimaryAntibodies}}