Anticorps Recombinant de lapin anti-Mmp9

Mmp9 Recombinant Antibody for IHC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

IHC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230069F1

N° de cat : 82854-5-PBS

Synonymes

230069F1, AW743869, B/MMP9, Clg4b, matrix metallopeptidase 9

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Galerie de données de validation

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  IHC staining of mouse spleen using 82854-5-RR (same clone as 82854-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 82854-5-RR (Mmp9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82854-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 82854-5-RR (same clone as 82854-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 82854-5-RR (Mmp9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82854-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 82854-5-RR (same clone as 82854-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 82854-5-RR (Mmp9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82854-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 82854-5-RR (same clone as 82854-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 82854-5-RR (Mmp9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82854-5-PBS in a different storage buffer formulation.

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  FC experiment of RAW 264.7 using 82854-5-RR (same clone as 82854-5-PBS)

  1x10^6 unstimulated or LPS and BFA treated RAW 264.7 cells were intracellularly stained with 0.13 ug Anti-Mouse Mmp9 (82854-5-RR, Clone:230069F1) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:700. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82854-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82854-5-RR

  Biolayer interferometry (BLl) kinetic assays of 82854-5-RR against Human Mmp9 were performed. The affinity constant is 76.6 pM.

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Informations sur le produit

82854-5-PBS cible Mmp9 dans les applications de IHC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Mmp9 Protéine recombinante Eg0452
• Nom complet: matrix metallopeptidase 9
• Masse moléculaire calculée: 81kd
• Poids moléculaire observé: 92-100 kDa
• Numéro d’acquisition GenBank: NM_013599
• Symbole du gène: Mmp9
• Identification du gène (NCBI): 17395
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Mmp9 (matrix metallopeptidase 9), also known as Clg4b. It is expected to be located inextracellular matrix. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, tissue remodeling, and disease processes, such as arthritis or metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. Studies in rhesus monkeys suggest that MMP9 is involved in IL-8-induced mobilization hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. The pro-MMP9 is 92 kDa, and it can be detected a processed form of 68 kDa. This protein can exist as a dimer of 180 kDa (PMID:7492685).

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