Anticorps Recombinant de lapin anti-Mmp9

Mmp9 Recombinant Antibody for IHC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

IHC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

230069F1

N° de cat : 82854-5-RR

Synonymes

230069F1, AW743869, B/MMP9, Clg4b, matrix metallopeptidase 9

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Galerie de données de validation

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  IHC staining of mouse spleen using 82854-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 82854-5-RR (Mmp9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 82854-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 82854-5-RR (Mmp9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 82854-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 82854-5-RR (Mmp9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 82854-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 82854-5-RR (Mmp9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of RAW 264.7 using 82854-5-RR

  1x10^6 unstimulated or LPS and BFA treated RAW 264.7 cells were intracellularly stained with 0.13 ug Anti-Mouse Mmp9 (82854-5-RR, Clone:230069F1) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:700. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 82854-5-RR

  Biolayer interferometry (BLl) kinetic assays of 82854-5-RR against Human Mmp9 were performed. The affinity constant is 76.6 pM.

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Applications testées

• Résultats positifs en IHC: tissu splénique de souris, tissu pulmonaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): 100ng/ml LPS+BFA RAW 264.7 cells

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82854-5-RR cible Mmp9 dans les applications de IHC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Mmp9 Protéine recombinante Eg0452
• Nom complet: matrix metallopeptidase 9
• Masse moléculaire calculée: 81kd
• Poids moléculaire observé: 92-100 kDa
• Numéro d’acquisition GenBank: NM_013599
• Symbole du gène: Mmp9
• Identification du gène (NCBI): 17395
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Mmp9 (matrix metallopeptidase 9), also known as Clg4b. It is expected to be located inextracellular matrix. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, tissue remodeling, and disease processes, such as arthritis or metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. Studies in rhesus monkeys suggest that MMP9 is involved in IL-8-induced mobilization hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. The pro-MMP9 is 92 kDa, and it can be detected a processed form of 68 kDa. This protein can exist as a dimer of 180 kDa (PMID:7492685).

Publications

Species	Application	Title
mouse	FC (Intra)	Commun Biol; The crosstalk of monocyte-neutrophil in hair follicles regulates neutrophil transepidermal migration in contact dermatitis; Authors - Zhan Fan

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