Anticorps Recombinant de lapin anti-Mmp9

Mmp9 Recombinant Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

230069G7

N° de cat : 82854-8-RR

Synonymes

230069G7, AW743869, B/MMP9, Clg4b, matrix metallopeptidase 9

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Galerie de données de validation

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  WB analysis using 82854-8-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82854-8-RR (Mmp9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 82854-8-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 82854-8-RR (Mmp9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 82854-8-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 82854-8-RR (Mmp9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 82854-8-RR

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 82854-8-RR (Mmp9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 82854-8-RR

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 82854-8-RR (Mmp9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 82854-8-RR

  Biolayer interferometry (BLl) kinetic assays of 82854-8-RR against Mouse Mmp9 were performed. The affinity constant is 1.38 nM.

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Applications testées

• Résultats positifs en WB: tissu splénique de souris, tissu pulmonaire de souris
• Résultats positifs en IHC: tissu splénique de souris, tissu pulmonaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

82854-8-RR cible Mmp9 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Mmp9 Protéine recombinante Eg0452
• Nom complet: matrix metallopeptidase 9
• Masse moléculaire calculée: 81kd
• Poids moléculaire observé: 92-100 kDa
• Numéro d’acquisition GenBank: NM_013599
• Symbole du gène: Mmp9
• Identification du gène (NCBI): 17395
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Mmp9 (matrix metallopeptidase 9), also known as Clg4b. It is expected to be located inextracellular matrix. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, tissue remodeling, and disease processes, such as arthritis or metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. Studies in rhesus monkeys suggest that MMP9 is involved in IL-8-induced mobilization hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. The pro-MMP9 is 92 kDa, and it can be detected a processed form of 68 kDa. This protein can exist as a dimer of 180 kDa (PMID:7492685).

Publications

Species	Application	Title
human	WB	Cell Death Dis; SP-1-activated LINC01016 overexpression promotes gastric cancer invasion and metastasis through inhibiting EIF4A3-mediated MMP9 mRNA decay; Authors - Ying Sun
mouse	IHC	Biochem Biophys Res Commun; Usefulness of serial in vivo imaging to directly assess the role of inflammation in thrombus resolution and organization; Authors - Aditya Adinata

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