Anticorps Recombinant de lapin anti-Moesin

• Phare
• Validé par KD/KO

Moesin Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3B8

N° de cat : 82009-1-PBS

Synonymes

MSN, Membrane-organizing extension spike protein, 3B8

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Galerie de données de validation

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  WB analysis using 82009-1-RR (same clone as 82009-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82009-1-RR (MSN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82009-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 82009-1-RR (same clone as 82009-1-PBS)

  WB result of Moesin antibody (82009-1-RR; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Moesin transfected HeLa cells. This data was developed using the same antibody clone with 82009-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 82009-1-RR (same clone as 82009-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82009-1-RR (Moesin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82009-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 82009-1-RR (same clone as 82009-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82009-1-RR (Moesin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82009-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 82009-1-RR (same clone as 82009-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82009-1-RR (Moesin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82009-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 82009-1-RR (same clone as 82009-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Moesin antibody (82009-1-RR, Clone: 3B8 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82009-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82009-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82009-1-RR against Human MSN were performed. The affinity constant is below 1 pM.

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Informations sur le produit

82009-1-PBS cible Moesin dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Moesin Protéine recombinante Ag9623
• Nom complet: moesin
• Masse moléculaire calculée: 577 aa, 68 kDa
• Poids moléculaire observé: 68-70 kDa
• Numéro d’acquisition GenBank: BC017293
• Symbole du gène: Moesin
• Identification du gène (NCBI): 4478
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Moesin belongs to the ezrin-radixin-moesin (ERM) family of proteins which act as cross-linkers between membrane and actin cytoskeleton. ERM proteins provide structural links to strengthen the cell cortex and facilitate several key cellular processes, including membrane dynamics, substrate adhesion, cell survival, cell adhesion, and motility. The function of ERM proteins is highly reliant on phosphorylation induced conformational changes in response to growth factor, chemokine, and antigen stimulation. This antibody may cross-react with ezrin or radixin with molecular weights around 68-70 kDa.

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