Anticorps Recombinant de lapin anti-Mono-Methyl-Histone H3 (Lys36)

Mono-Methyl-Histone H3 (Lys36) Recombinant Antibody for WB, IF/ICC, FC (Intra), Dot Blot, ELISA, ChIP-qPCR

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, FC (Intra), Dot Blot, ELISA, ChIP-qPCR

Conjugaison

Non conjugué

CloneNo.

241138B6

N° de cat : 82825-2-RR

Synonymes

H3 Me Lys36, H3 MeK36, H3 Methyl K36, H3 Methyl Lys36, H3 Methyl Lysine36

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Galerie de données de validation

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  WB analysis using 82825-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82825-2-RR (HIST1H3A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 82825-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Mono-Methyl-Histone H3 (Lys36) antibody (82825-2-RR, Clone: 241138B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of HeLa using 82825-2-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Hist1h3a Recombinant Antibody (82825-2-RR, Clone:241138B6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  Dot Blot experiment of peptide using 82825-2-RR

  Dot blot analysis was used to confirm the specificity of Mono-Methyl-Histone H3 (Lys36) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.

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  ChIP experiment of HeLa using 82825-2-RR

  Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Mono-Methyl-Histone H3 (Lys36) (82825-2-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HeLa, cellules HSC-T6, cellules K-562, cellules NIH/3T3, cellules THP-1, tissu hépatique de souris
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules HeLa,
• Résultats positifs en Dot Blot: Peptide,
• Résultats positifs en ChIP-qPCR: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
• DOT BLOT: DOT BLOT : 1:10-1:100
• CHImmunoprécipitation (IP)-QPCR: CHIP-QPCR : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82825-2-RR cible Mono-Methyl-Histone H3 (Lys36) dans les applications de WB, IF/ICC, FC (Intra), Dot Blot, ELISA, ChIP-qPCR et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: histone cluster 1, H3a
• Poids moléculaire observé: 15 kDa
• Numéro d’acquisition GenBank: BC066245
• Symbole du gène: HIST1H3A
• Identification du gène (NCBI): 8350
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Histones, including H1/H5 (linker histones), H2, H3, and H4 (core histones), are nucleic proteins which interact with DNA to form the nucleosomes and play important roles in gene regulation and DNA replication. Histone proteins are highly post-translationally modified while Histone H3 is the most extensively modified.

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