Anticorps Recombinant de lapin anti-CD45.1

CD45.1 Recombinant Antibody for WB, IF-Fro, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, IF-Fro, ELISA

Conjugaison

Non conjugué

CloneNo.

240921B5

N° de cat : 84325-1-RR

Synonymes

Ly5a, Cd45, Ptprc, 240921B5, B220

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Galerie de données de validation

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  WB analysis using 84325-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84325-1-RR (Mouse CD45.1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse spleen using 84325-1-RR

  Immunofluorescent analysis of un-fixed frozen OCT-embedded mouse spleen tissue using CD45.1 antibody (84325-1-RR, Clone: 240921B5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: SJL mouse thymus tissue, SJL mouse spleen tissue
• Résultats positifs en IF-Fro: tissu splénique de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)-FRO: IF-FRO : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84325-1-RR cible CD45.1 dans les applications de WB, IF-Fro, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: protein tyrosine phosphatase, receptor type, C
• Poids moléculaire observé: 220 kDa
• Symbole du gène: CD45
• Identification du gène (NCBI): 19264
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). Allelic variants of mouse CD45, CD45.1 (Ly5.1) and CD45.2 (Ly5.1), have been established as a marker system to track haematopoietic cells following congenic mouse bone marrow transplants (PMID: 28615666). CD45.2 is the common form and is expressed by most of the established strains, while CD45.1 is found in only a few like the SJL mouse strain (PMID: 3489673).

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