Anticorps Monoclonal anti-Mouse IgG2b isotype control

Mouse IgG2b isotype control Monoclonal Antibody for WB, IHC, FC, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Megathura crenulata

Applications

WB, IHC, FC, IP, chIP, ELISA

Conjugaison

Non conjugué

Publications(7)

CloneNo.

11B8C4

N° de cat : 66360-3-Ig

Synonymes

Mouse IgG2b

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• Galerie de données de validation
• Proteintech vs. Other Vendors

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  WB analysis using 66360-3-Ig

  Various lysates (50 ug/lane) were subjected to SDS PAGE followed by western blot with 66360-3-Ig (Mouse IgG2b isotype control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 66360-3-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66360-3-Ig (Mouse IgG2b isotype control antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 66360-3-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66360-3-Ig (Mouse IgG2b isotype control antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of human PBMCs using 66360-3-Ig

  1x10^6 human PBMCs were surface stained with 0.5 ug Mouse IgG2b isotype control Monoclonal antibody (66360-3-Ig, Clone: 11B8C4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1) (red), or 0.5 ug Anti-Human CD20 (65085-1-Ig) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1) (green), or unstained (blue). Cells were not fixed.

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Applications testées

• Résultats positifs en IHC: il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC: CMSP humaines,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:1500-1:6000
• Flow Cytometry (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IP: See 1 publications below
• ChIP: See 2 publications below

Informations sur le produit

66360-3-Ig cible Mouse IgG2b isotype control dans les applications de WB, IHC, FC, IP, chIP, ELISA et montre une réactivité avec des échantillons Megathura crenulata

• Réactivité: Megathura crenulata
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: Mouse IgG2b isotype control
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

This antibody is used as the isotype control of mouse IgG2b. It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).

Publications

Species	Application	Title
	ChIP	Front Oncol; The COX10-AS1/miR-641/E2F6 Feedback Loop Is Involved in the Progression of Glioma.; Authors - Liang Liu
	IP	Sci Rep; Premature termination codon readthrough in Drosophila varies in a developmental and tissue-specific manner.; Authors - Yanan Chen
	chIP	Cell Death Dis; E2F1 mediated DDX11 transcriptional activation promotes hepatocellular carcinoma progression through PI3K/AKT/mTOR pathway.; Authors - Yan Yu
	WB	Aging Dis; Age-related Loss of miR-124 Causes Cognitive Deficits via Derepressing RyR3 Expression; Authors - Kai Liu
		Immunity; Ribosome-rescuer PELO catalyzes the oligomeric assembly of NOD-like receptor family proteins via activating their ATPase enzymatic activity; Authors - Xiurong Wu
		Nat Commun; Generation of nanobodies from transgenic 'LamaMice' lacking an endogenous immunoglobulin repertoire; Authors - Thomas Eden

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