Anticorps Monoclonal anti-CD16

CD16 Monoclonal Antibody for Single Cell (Intra), Single Cell

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain

Applications

Single Cell (Intra), Single Cell

Conjugaison

5CFLX Fluorescent Dye

CloneNo.

3G8

N° de cat : G65090-1-5C

Synonymes

CD16, CD16A, CD16a antigen, CD16B, CD16b antigen, Fc gamma RIII, Fc gamma RIII alpha, Fc gamma RIIIa, FCG3, FCGR3, FCGR3A, FCGRIII, FCR 10, FCRIII, FCRIIIA, IGFR3, IgG Fc receptor III 2

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Galerie de données de validation

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  Single Cell Sequencing experiment G65090-1-5C on Resting PBMC

  G65090-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

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  Single Cell Sequencing experiment G65090-1-5C on PBMC treated with 4hr LPS + TI

  G65090-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

• 

  Single Cell Sequencing experiment G65090-1-5C on Resting PBMC

  G65090-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

• 

  Single Cell Sequencing experiment G65090-1-5C on PBMC treated with 4hr LPS + TI

  G65090-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

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Applications testées

• Résultats positifs en Single Cell (Intra): 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.
• Résultats positifs en Single Cell: 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.

Dilution recommandée

• SINGLE CELL (INTRA): SINGLE CELL (INTRA) : <0.5ug/test
• SINGLE CELL: SINGLE CELL : <0.5ug/test

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

G65090-1-5C cible CD16 dans les applications de Single Cell (Intra), Single Cell et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: s/o
• Nom complet: Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
• Masse moléculaire calculée: 254 aa, 29 kDa
• Numéro d’acquisition GenBank: BC017865
• Symbole du gène: CD16a
• Identification du gène (NCBI): 2214
• Conjugaison: 5CFLX Fluorescent Dye
• Forme: Liquide
• Tampon de stockage: PBS with 1mM EDTA and 0.09% sodium azide
• Conditions de stockage: 2-8°C Stable for one year after shipment. 20ul contiennent 0,1% de BSA.

Informations générales

CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B. Clone 3G8 recognizes both the CD16a and CD16b (PMID: 7592758).

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