Anticorps Monoclonal anti-CD3

CD3 Monoclonal Antibody for Single Cell (Intra)

Hôte / Isotype

Mouse / IgG2a, kappa

Réactivité testée

Humain

Applications

Single Cell (Intra)

Conjugaison

5CFLX Fluorescent Dye

CloneNo.

OKT3

N° de cat : G65133-1-5C

Synonymes

CD3, CD3E, T3E, TCRE

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Galerie de données de validation

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  Single Cell Sequencing experiment G65133-1-5C on Resting PBMC

  G65133-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

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  Single Cell Sequencing experiment G65133-1-5C on PBMC treated with 4hr LPS + TI

  G65133-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

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Applications testées

• Résultats positifs en Single Cell (Intra): 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.

Dilution recommandée

• SINGLE CELL (INTRA): SINGLE CELL (INTRA) : <0.5ug/test

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

G65133-1-5C cible CD3 dans les applications de Single Cell (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Human T cells
• Nom complet: CD3e molecule, epsilon (CD3-TCR complex)
• Masse moléculaire calculée: 207 aa, 23 kDa
• Numéro d’acquisition GenBank: BC049847
• Symbole du gène: CD3
• Identification du gène (NCBI): 916
• Conjugaison: 5CFLX Fluorescent Dye
• Forme: Liquide
• Tampon de stockage: PBS with 1mM EDTA and 0.09% sodium azide
• Conditions de stockage: 2-8°C Stable for one year after shipment. 20ul contiennent 0,1% de BSA.

Informations générales

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

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