Anticorps Polyclonal de lapin anti-LEF1

LEF1 Polyclonal Antibody for Single Cell (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

Single Cell (Intra)

Conjugaison

5CFLX Fluorescent Dye

N° de cat : G14972-1-5C

Synonymes

LEF 1, LEF1, TCF1 alpha, TCF1ALPHA

---

Galerie de données de validation

• 

  Single Cell Sequencing experiment G14972-1-5C on Resting PBMC

  G14972-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

• 

  Single Cell Sequencing experiment G14972-1-5C on PBMC treated with 4hr LPS + TI

  G14972-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

---

Applications testées

• Résultats positifs en Single Cell (Intra): 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.

Dilution recommandée

• SINGLE CELL (INTRA): SINGLE CELL (INTRA) : <0.5ug/test

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

G14972-1-5C cible LEF1 dans les applications de Single Cell (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LEF1 Protéine recombinante Ag6882
• Nom complet: lymphoid enhancer-binding factor 1
• Masse moléculaire calculée: 37 kDa
• Numéro d’acquisition GenBank: BC050632
• Symbole du gène: LEF1
• Identification du gène (NCBI): 51176
• Conjugaison: 5CFLX Fluorescent Dye
• Forme: Liquide
• Tampon de stockage: PBS with 1mM EDTA and 0.09% sodium azide
• Conditions de stockage: 2-8°C Stable for one year after shipment. 20ul contiennent 0,1% de BSA.

Informations générales

Lymphoid enhancer-binding factor 1(LEF1) belongs to a family of regulatory protein share homology with high mobility group protein-1, and it's a nuclear protein exprssed in pre-B and T cells. LEF1 has a role in the Wnt signaling pathway and hair cell differentiation and follicle morphogenesis. Together with CTNNB1 and EP300, LEF1 activates transcription of target genes. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells. MECs can give rise to seven cell types of the SAE and SMGs following severe airway injury. MECs progressively adopted a basal cell phenotype on the SAE and established lasting progenitors capable of further regeneration following reinjury. MECs activate Wnt-regulated transcription factors (Lef-1/TCF7) following injury and Lef-1 induction in cultured MECs promoted transition to a basal cell phenotype. Surprisingly, dose-dependent MEC conditional activation of Lef-1in vivopromoted self-limited airway regeneration in the absence of injury. Thus, modulating the Lef-1 transcriptional program in MEC-derived progenitors may have regenerative medicine applications for lung diseases. (https://doi.org/10.1016/j.stem.2018.03.017) The phosphorylation may affects LEF1 protein's theoretical molecular weight when tested.40-70 kD bands have also been reported (PMID: 22261717; 17063141 ).

{{ptg:RelatedPrimaryAntibodies}}