Anticorps Monoclonal anti-TNF Alpha

TNF Alpha Monoclonal Antibody for Single Cell (Intra)

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

Single Cell (Intra)

Conjugaison

5CFLX Fluorescent Dye

CloneNo.

7B8A11

N° de cat : G60291-1-5C

Synonymes

Cachectin, DIF, TNF, TNF a, TNF alpha, TNFA, TNF-a, TNF-alpha, TNFSF2, TNFα, TNF-α, Tumor necrosis factor

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Galerie de données de validation

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  Single Cell Sequencing experiment G60291-1-5C on Resting PBMC

  G60291-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

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  Single Cell Sequencing experiment G60291-1-5C on PBMC treated with 4hr LPS + TI

  G60291-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

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Applications testées

• Résultats positifs en Single Cell (Intra): 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.

Dilution recommandée

• SINGLE CELL (INTRA): SINGLE CELL (INTRA) : <0.5ug/test

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

G60291-1-5C cible TNF Alpha dans les applications de Single Cell (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TNF Alpha Protéine recombinante Ag11413
• Nom complet: tumor necrosis factor (TNF superfamily, member 2)
• Masse moléculaire calculée: 233 aa, 26 kDa
• Numéro d’acquisition GenBank: BC028148
• Symbole du gène: TNF-alpha
• Identification du gène (NCBI): 7124
• Conjugaison: 5CFLX Fluorescent Dye
• Forme: Liquide
• Tampon de stockage: PBS with 1mM EDTA and 0.09% sodium azide
• Conditions de stockage: 2-8°C Stable for one year after shipment. 20ul contiennent 0,1% de BSA.

Informations générales

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, INS resistance, and cancer.

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