Anticorps Polyclonal de lapin anti-Vimentin

Vimentin Polyclonal Antibody for Single Cell (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

Single Cell (Intra)

Conjugaison

5CFLX Fluorescent Dye

N° de cat : G10366-1-5C

Synonymes

VIM, vimentin

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Galerie de données de validation

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  Single Cell Sequencing experiment G10366-1-5C on Resting PBMC

  G10366-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

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  Single Cell Sequencing experiment G10366-1-5C on PBMC treated with 4hr LPS + TI

  G10366-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

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Applications testées

• Résultats positifs en Single Cell (Intra): 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.

Dilution recommandée

• SINGLE CELL (INTRA): SINGLE CELL (INTRA) : <0.5ug/test

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

G10366-1-5C cible Vimentin dans les applications de Single Cell (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Vimentin Protéine recombinante Ag0489
• Nom complet: vimentin
• Masse moléculaire calculée: 466 aa, 54 kDa
• Numéro d’acquisition GenBank: BC000163
• Symbole du gène: VIM
• Identification du gène (NCBI): 7431
• Conjugaison: 5CFLX Fluorescent Dye
• Forme: Liquide
• Tampon de stockage: PBS with 1mM EDTA and 0.09% sodium azide
• Conditions de stockage: 2-8°C Stable for one year after shipment. 20ul contiennent 0,1% de BSA.

Informations générales

Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level. Isoforms of vimentin (49 kDa and 60 kDa) had also been reported. (PMID: 8640945, 22728585).

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