Anticorps Polyclonal de lapin anti-MBP

MBP Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, IF-Fro, ELISA

Conjugaison

Non conjugué

N° de cat : 10458-1-PBS

Synonymes

myelin basic protein

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Galerie de données de validation

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  WB analysis using 10458-1-AP (same clone as 10458-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10458-1-AP (Myelin basic protein antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10458-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 10458-1-AP (same clone as 10458-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10458-1-AP (Myelin basic protein antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10458-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 10458-1-AP (same clone as 10458-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10458-1-AP (Myelin basic protein antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10458-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 10458-1-AP (same clone as 10458-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Myelin basic protein antibody (10458-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10458-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 10458-1-AP (same clone as 10458-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using Myelin basic protein antibody (10458-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 10458-1-PBS in a different storage buffer formulation.

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Informations sur le produit

10458-1-PBS cible MBP dans les applications de WB, IHC, IF-P, IF-Fro, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MBP Protéine recombinante Ag0713
• Nom complet: myelin basic protein
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 14-23 kDa
• Numéro d’acquisition GenBank: BC008749
• Symbole du gène: Myelin basic protein
• Identification du gène (NCBI): 4155
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MBP belongs to the myelin basic protein family. The classic group of MBP isoforms (isoform 4-isoform 14) are the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. MBP has six isoforms. Catalog#10458-1-AP is capable of recognizing multiple isoforms of MBP.

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