Anticorps Recombinant de lapin anti-MYOF

• Phare
• Validé par KD/KO

MYOF Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

230213E10

N° de cat : 82917-2-RR

Synonymes

Myoferlin, 230213E10, Fer 1 like family member 3, Fer 1 like protein 3, FER1L3

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Galerie de données de validation

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  WB analysis using 82917-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82917-2-RR (Myoferlin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 82917-2-RR

  WB result of MYOF antibody (82917-2-RR; 1:200000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MYOF transfected A549 cells.

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  IF Staining of HepG2 using 82917-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MYOF antibody (82917-2-RR, Clone: 230213E10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of HeLa using 82917-2-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug MYOF Recombinant antibody (82917-2-RR, Clone:230213E10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules U-251
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82917-2-RR cible MYOF dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: myoferlin
• Masse moléculaire calculée: 235 kDa
• Poids moléculaire observé: 230-240 kDa
• Numéro d’acquisition GenBank: NM_133337
• Symbole du gène: MYOF
• Identification du gène (NCBI): 26509
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MYOF, also named FER1L3 and KIAA1207, belongs to the Ferlin family. MYOF is a calcium/phospholipid-binding protein that plays a role in the plasmalemma repair mechanism of endothelial cells that permits rapid resealing of membranes disrupted by mechanical stress. It is involved in endocytic recycling. MYOF is implicated in VEGF signal transduction by regulating the levels of the receptor KDR.

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