Anticorps Monoclonal anti-Myogenin

Myogenin Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat et plus (4)

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

13

CloneNo.

1C10B9

N° de cat : 67082-1-Ig

Synonymes

bHLHc3, Myf 4, MYF4, MYOG, Myogenic factor 4, MYOGENIN, myogenin (myogenic factor 4)

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Galerie de données de validation

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  WB analysis of human skeletal muscle using 67082-1-Ig

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 67082-1-Ig (Myogenin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 67082-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 67082-1-Ig (Myogenin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig heart using 67082-1-Ig

  pig heart tissue were subjected to SDS PAGE followed by western blot with 67082-1-Ig (Myogenin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 67082-1-Ig

  rat heart tissue were subjected to SDS PAGE followed by western blot with 67082-1-Ig (Myogenin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu de muscle squelettique humain, tissu cardiaque de porc, tissu cardiaque de rat, tissu cardiaque humain

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 13 publications below
• IF: See 3 publications below

Informations sur le produit

67082-1-Ig cible Myogenin dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, rat

• Réactivité: Humain, porc, rat
• Réactivité citée: bovin, Chèvre, Humain, Lapin, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Myogenin Protéine recombinante Ag6414
• Nom complet: myogenin (myogenic factor 4)
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 26 kDa
• Numéro d’acquisition GenBank: BC053899
• Symbole du gène: MYOG
• Identification du gène (NCBI): 4656
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MYOG (encoded Myogenin protein) is a muscle-specific transcription factor which belongs to a family of basic helix-loop-helix transcription factors. It plays a role in muscle differentiation, cell cycle exit and muscle atrophy(PMID: 30315160). It can be initiate the myogenic program in myoblasts, fiblasts and veritey of other cell types. Diseases associated with MYOG include Embryonal Rhabdomyosarcoma and Gallbladder Sarcoma.

Publications

Species	Application	Title
mouse	WB	J Cachexia Sarcopenia Muscle; circRNAome profiling reveals circFgfr2 regulates myogenesis and muscle regeneration via a feedback loop.; Authors - Junyu Yan
human	WB	Mol Ther Nucleic Acids; Circular RNA RBPMS inhibits bladder cancer progression via miR-330-3p/RAI2 regulation.; Authors - Chen Yang
mouse	WB	Int J Mol Sci; G6PD Deficiency Is Crucial for Insulin Signaling Activation in Skeletal Muscle.; Authors - Aiwen Jiang
mouse	WB	Front Cell Dev Biol; Phosphorylation of Dynamin-Related Protein 1 (DRP1) Regulates Mitochondrial Dynamics and Skeletal Muscle Wasting in Cancer Cachexia.; Authors - Xiangyu Mao
goat	WB	FASEB J; Circular RNA circUSP13 sponges miR-29c to promote differentiation and inhibit apoptosis of goat myoblasts by targeting IGF1.; Authors - Zhen Zhang
	WB	FASEB J; MicroRNA profiling reveals miR-145-5p inhibits goat myoblast differentiation by targeting the coding domain sequence of USP13.; Authors - Zhen Zhang