Anticorps Monoclonal anti-Myoglobin

Myoglobin Monoclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1A4F7

N° de cat : 66205-1-PBS

Synonymes

MB, 1A4F7, EC:1.11.1.-, EC:1.7.-.-, Nitrite reductase MB

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Galerie de données de validation

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  WB analysis of human skeletal muscle using 66205-1-Ig (same clone as 66205-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66205-1-Ig (Myoglobin antibody at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66205-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 66205-1-Ig (same clone as 66205-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 66205-1-Ig (Myoglobin antibody at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66205-1-PBS in a different storage buffer formulation.

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  WB analysis of pig heart using 66205-1-Ig (same clone as 66205-1-PBS)

  pig heart tissue were subjected to SDS PAGE followed by western blot with 66205-1-Ig (Myoglobin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66205-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat heart using 66205-1-Ig (same clone as 66205-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 66205-1-Ig (Myoglobin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66205-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse heart using 66205-1-Ig (same clone as 66205-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 66205-1-Ig (Myoglobin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66205-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rabbit heart using 66205-1-Ig (same clone as 66205-1-PBS)

  rabbit heart tissue were subjected to SDS PAGE followed by western blot with 66205-1-Ig (Myoglobin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66205-1-PBS in a different storage buffer formulation.

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  IHC staining of rat heart using 66205-1-Ig (same clone as 66205-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66205-1-Ig (Myoglobin antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66205-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 66205-1-Ig (same clone as 66205-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66205-1-Ig (Myoglobin antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66205-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse tongue using 66205-1-Ig (same clone as 66205-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse tongue tissue slide using 66205-1-Ig (Myoglobin antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66205-1-PBS in a different storage buffer formulation.

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  FC experiment of C2C12 using 66205-1-Ig (same clone as 66205-1-PBS)

  1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human Myoglobin (66205-1-Ig, Clone:1A4F7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66205-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66205-1-PBS cible Myoglobin dans les applications de WB, IHC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Myoglobin Protéine recombinante Ag8979
• Nom complet: myoglobin
• Masse moléculaire calculée: 154 aa, 17 kDa
• Poids moléculaire observé: 17-18 kDa
• Numéro d’acquisition GenBank: BC014547
• Symbole du gène: Myoglobin
• Identification du gène (NCBI): 4151
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Myoglobin is a cytoplasmic hemoprotein that is expressed primarily in cardiomyocytes and oxidative skeletal muscle fibers, functioning on facilitating oxygen transport and modulating nitric oxide homeostasis within cardiac and skeletal myocytes. Recent studies indicated that myoglobin was also expressed in non-muscle tissues. This antibody well recognized endogenous myoglobin in muscle lysates.

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