Anticorps Monoclonal anti-Myosin Light Chain 2/MLC-2V

Myosin Light Chain 2/MLC-2V Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

5A1F1

N° de cat : 60229-1-PBS

Synonymes

MLC 2v, MYL2, MLC-2s/v, MLC-2, MLC2

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Galerie de données de validation

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  WB analysis using 60229-1-Ig (same clone as 60229-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60229-1-Ig (Myosin Light Chain 2/MLC-2V antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60229-1-PBS in a different storage buffer formulation.

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  WB analysis of pig heart using 60229-1-Ig (same clone as 60229-1-PBS)

  pig heart tissue were subjected to SDS PAGE followed by western blot with 60229-1-Ig (Myosin Light Chain 2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60229-1-PBS in a different storage buffer formulation.

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  WB analysis of rat heart using 60229-1-Ig (same clone as 60229-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 60229-1-Ig (Myosin Light Chain 2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60229-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 60229-1-Ig (same clone as 60229-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60229-1-Ig (Myosin Light Chain 2 antibody) at dilution of 1:500 (under 0x lens). This data was developed using the same antibody clone with 60229-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 60229-1-Ig (same clone as 60229-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60229-1-Ig (Myosin Light Chain 2 antibody) at dilution of 1:500 (under 0x lens). This data was developed using the same antibody clone with 60229-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 60229-1-Ig (same clone as 60229-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 60229-1-Ig (Myosin Light Chain 2/MLC-2V antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60229-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 60229-1-Ig (same clone as 60229-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 60229-1-Ig (Myosin Light Chain 2/MLC-2V antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60229-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 60229-1-Ig (same clone as 60229-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60229-1-Ig (Myosin Light Chain 2 antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 60229-1-PBS in a different storage buffer formulation.

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  IF Staining of C2C12 using 60229-1-Ig (same clone as 60229-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using Myosin Light Chain 2/MLC-2V antibody (60229-1-Ig, Clone: 5A1F1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60229-1-PBS in a different storage buffer formulation.

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Informations sur le produit

60229-1-PBS cible Myosin Light Chain 2/MLC-2V dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Myosin Light Chain 2/MLC-2V Protéine recombinante Ag1356
• Nom complet: myosin, light chain 2, regulatory, cardiac, slow
• Masse moléculaire calculée: 19 kDa
• Poids moléculaire observé: 18 kDa
• Numéro d’acquisition GenBank: BC015821
• Symbole du gène: Myosin Light Chain 2
• Identification du gène (NCBI): 4633
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MYL2, also named as MLC-2v and MLC-2, is ventricular/cardiac muscle isoform. Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic type 10 (CMH10). Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 2 (MVC2). MYL2 has been widely used as a marker of mature ventricular cardiomyocytes.

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