Anticorps Polyclonal de lapin anti-N-cadherin

N-cadherin Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(56)

N° de cat : 13769-1-AP

Synonymes

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Galerie de données de validation

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  WB analysis of HEK-293 using 13769-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13769-1-AP (N-cadherin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 13769-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13769-1-AP (N-cadherin Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 13769-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13769-1-AP (N-cadherin Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules C6
• Résultats positifs en IHC: tissu cardiaque humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 43 publications below
• IHC: See 9 publications below
• IF: See 6 publications below

Informations sur le produit

13769-1-AP cible N-cadherin dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: N-cadherin Protéine recombinante Ag4752
• Nom complet: cadherin 2, type 1, N-cadherin (neuronal)
• Masse moléculaire calculée: 906 aa, 100 kDa
• Poids moléculaire observé: 90 kDa, 140 kDa
• Numéro d’acquisition GenBank: BC036470
• Symbole du gène: N-cadherin
• Identification du gène (NCBI): 1000
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a classical member of the cadherin superfamily which also include E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes (PMID: 11282032; 9508779; 8125202). N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity (PMID: 23321619).

Publications

Species	Application	Title
		Biomaterials; Comparative effects of N-cadherin protein and peptide fragments on mesenchymal stem cell mechanotransduction and paracrine function.; Authors - Ellen C Qin
human	WB,IF	Theranostics; Gas6/Axl Axis Contributes to Chemoresistance and Metastasis in Breast Cancer through Akt/GSK-3β/β-catenin Signaling.; Authors - Cun Wang
mouse	WB	Int J Cancer; Plant galactolipid dLGG suppresses lung metastasis of melanoma through deregulating TNF-α-mediated pulmonary vascular permeability and circulating oxylipin dynamics in mice.; Authors - Chung-Chih Yang
mouse	WB	Oxid Med Cell Longev; Metformin Improves Fertility in Obese Males by Alleviating Oxidative Stress-Induced Blood-Testis Barrier Damage.; Authors - Jifeng Ye
human	WB,IHC	Oncotarget; microRNA-374a suppresses colon cancer progression by directly reducing CCND1 to inactivate the PI3K/AKT pathway.; Authors - Yiyu Chen
human	WB	Cancer Lett; Disrupting MALAT1/miR-200c sponge decreases invasion and migration in endometrioid endometrial carcinoma.; Authors - Qiulian Li

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