Anticorps Monoclonal anti-N-cadherin

N-cadherin Monoclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

2G7C2

N° de cat : 68532-1-Ig

Synonymes

Cadherin 2, CD325, CDH2, CDHN, CDw325, N cadherin, NCAD, Neural cadherin

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Galerie de données de validation

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  WB analysis using 68532-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68532-1-Ig (N-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of hTERT-RPE1 using 68532-1-Ig

  hTERT-RPE1 cells were subjected to SDS PAGE followed by western blot with 68532-1-Ig (N-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 68532-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68532-1-Ig (N-cadherin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 68532-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68532-1-Ig (N-cadherin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse heart using 68532-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using N-cadherin antibody (68532-1-Ig, Clone: 2G7C2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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  IF Staining of mouse heart using 68532-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using N-cadherin antibody (68532-1-Ig, Clone: 2G7C2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de porc, cellules hTERT-RPE1, tissu cardiaque de lapin, tissu cardiaque de rat, tissu cardiaque de souris
• Résultats positifs en IHC: tissu cardiaque de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cardiaque de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68532-1-Ig cible N-cadherin dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: cadherin 2, type 1, N-cadherin (neuronal)
• Masse moléculaire calculée: 100 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: NM_001792.4
• Symbole du gène: N-cadherin
• Identification du gène (NCBI): 1000
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a 130-kDa transmembrane protein and a classical member of the cadherin superfamily which also include E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes (PMID: 11282032; 9508779; 8125202). N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity (PMID: 23321619). The N-cadherin ectodomain can be cleaved, leading to the generation of a 90 kD N-terminal fragment (PMID: 16998833; 17028923).

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