Anticorps Monoclonal anti-N-cadherin

N-cadherin Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2G7C2

N° de cat : 68532-1-PBS

Synonymes

Cadherin 2, CD325, CDH2, CDHN, CDw325, N cadherin, NCAD, Neural cadherin

---

Galerie de données de validation

• 

  WB analysis using 68532-1-Ig (same clone as 68532-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68532-1-Ig (N-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68532-1-PBS in a different storage buffer formulation.

• 

  WB analysis of hTERT-RPE1 using 68532-1-Ig (same clone as 68532-1-PBS)

  hTERT-RPE1 cells were subjected to SDS PAGE followed by western blot with 68532-1-Ig (N-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68532-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 68532-1-Ig (same clone as 68532-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68532-1-Ig (N-cadherin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68532-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 68532-1-Ig (same clone as 68532-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68532-1-Ig (N-cadherin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68532-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse heart using 68532-1-Ig (same clone as 68532-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using N-cadherin antibody (68532-1-Ig, Clone: 2G7C2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68532-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse heart using 68532-1-Ig (same clone as 68532-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using N-cadherin antibody (68532-1-Ig, Clone: 2G7C2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68532-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

68532-1-PBS cible N-cadherin dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: cadherin 2, type 1, N-cadherin (neuronal)
• Masse moléculaire calculée: 100 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: NM_001792.4
• Symbole du gène: N-cadherin
• Identification du gène (NCBI): 1000
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a 130-kDa transmembrane protein and a classical member of the cadherin superfamily which also include E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes (PMID: 11282032; 9508779; 8125202). N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity (PMID: 23321619). The N-cadherin ectodomain can be cleaved, leading to the generation of a 90 kD N-terminal fragment (PMID: 16998833; 17028923).

{{ptg:RelatedPrimaryAntibodies}}