Anticorps Monoclonal anti-N-cadherin

N-cadherin Monoclonal Antibody for WB, IF/ICC, FC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IF/ICC, FC, ELISA

Conjugaison

Non conjugué

CloneNo.

2D9G1

N° de cat : 68532-2-Ig

Synonymes

2D9G1, Cadherin 2, Cadherin-2, CD325, CDH2

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Galerie de données de validation

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  WB analysis using 68532-2-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68532-2-Ig (N-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 68532-2-Ig

  Immunofluorescent analysis of un-fixed HepG2 cells using N-cadherin antibody (68532-2-Ig, Clone: 2D9G1 ) at dilution of 1:500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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  FC experiment of HepG2 using 68532-2-Ig

  1x10^6 HepG2 cells were surface stained with 0.2 μg N-Cadherin Monoclonal Antibody (68532-2-Ig, Clone: 2D9G1, red) or 0.2 μg Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue), and CoraLite488-conjugated Goat Anti-Mouse IgG(H+L) (Cat.NO. SA00013-1). Cells were not fixed.

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  FC experiment of SH-SY5Y using 68532-2-Ig

  1x10^6 MCF-7 (red, dashed) and SH-SY5Y (red, filled) were surface stained with 0.2 μg N-cadherin Monoclonal antibody (68532-2-Ig, Clone: 2D9G1) and CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(Cat.NO.RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue) was parallel stained as control. Cells were not fixed.

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  Affinity and Kinetic Characterization of 68532-2-Ig

  Biolayer interferometry (BLl) kinetic assays of 68532-2-PBS against Human N-cadherin were performed. The affinity constant is 2.69 nM.

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Applications testées

• Résultats positifs en WB: tissu cardiaque de porc, tissu cardiaque de lapin, tissu cardiaque de rat, tissu cardiaque de souris
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en cytométrie: cellules SH-SY5Y, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68532-2-Ig cible N-cadherin dans les applications de WB, IF/ICC, FC, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: N-cadherin Protéine recombinante Eg0701
• Nom complet: cadherin 2, type 1, N-cadherin (neuronal)
• Masse moléculaire calculée: 100 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: NM_001792.4
• Symbole du gène: N-cadherin
• Identification du gène (NCBI): 1000
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a 130-kDa transmembrane protein and a classical member of the cadherin superfamily which also include E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes (PMID: 11282032; 9508779; 8125202). N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity (PMID: 23321619). The N-cadherin ectodomain can be cleaved, leading to the generation of a 90 kD N-terminal fragment (PMID: 16998833; 17028923).

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