Anticorps Recombinant de lapin anti-N-cadherin

• Phare
• Validé par KD/KO

N-cadherin Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

230259H5

N° de cat : 82968-1-RR

Synonymes

230259H5, Cadherin 2, Cadherin-2, CD325, CDH2

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Galerie de données de validation

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  WB analysis of HEK-293 using 82968-1-RR

  WB result of N-cadherin antibody (82968-1-RR; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-N-cadherin transfected HEK-293 cells.

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  WB analysis using 82968-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82968-1-RR (N-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 82968-1-RR

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82968-1-RR (N-cadherin antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 82968-1-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 82968-1-RR (N-cadherin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 82968-1-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 82968-1-RR (N-cadherin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 82968-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using N-cadherin antibody (82968-1-RR, Clone: 230259H5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of A549 using 82968-1-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using N-cadherin antibody (82968-1-RR, Clone: 230259H5 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  IF Staining of HeLa using 82968-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using N-cadherin antibody (82968-1-RR, Clone: 230259H5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HeLa using 82968-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using N-cadherin antibody (82968-1-RR, Clone: 230259H5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 82968-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82968-1-RR against Human N-cadherin were performed. The affinity constant is 0.464 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293
• Résultats positifs en IHC: human intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A549, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82968-1-RR cible N-cadherin dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: N-cadherin Protéine recombinante Eg0701
• Nom complet: cadherin 2, type 1, N-cadherin (neuronal)
• Masse moléculaire calculée: 100 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: NM_001792.4
• Symbole du gène: N-cadherin
• Identification du gène (NCBI): 1000
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a 130-kDa transmembrane protein and a classical member of the cadherin superfamily which also includes E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes (PMID: 11282032; 9508779; 8125202). N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity (PMID: 23321619). The N-cadherin ectodomain can be cleaved, leading to the generation of a 90 kD N-terminal fragment (PMID: 16998833; 17028923).

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