Anticorps Monoclonal anti-N4-acetylcytidine

N4-acetylcytidine Monoclonal Antibody for Dot Blot, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

chemical compound et plus (1)

Applications

RIP, Dot Blot, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

3C2C2

N° de cat : 68498-1-Ig

Synonymes

C11H15N3O6, Ac-rC, Acetylcytidine, ACETYL CYTOSINE, Ac4C

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Galerie de données de validation

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  ELISA experiment of N4-acetylcytidine using 68498-1-Ig

  Indirect ELISA and competitive ELISA results show that this antibody is specific to ac4C (N4-acetylcytidine). Indirect ELISA (blue curve, refer to top X-right Y axis) was performed by coating BSA conjugated ac4C at 50ng/well followed by blocking with 5% non fat milk. Serial diluted primary antibody was added to the plates and incubated at 37℃. HRP-goat anti-mouse was used for detection. Competitive ELISA was performed similarly except that different concentration of Ac4C or its structure analogue compounds was mixed in 20ng/mL primary antibody (red curve, refer to bottom X-left Y axis).

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  Dot Blot experiment of HeLa using 68498-1-Ig

  Total RNA was isolated from HeLa cell line and was dotted to NC membrane at different amount as indicated above the dots. The membrane was blocked with 5% milk and blotted with N4-acetylcytidine antibody 68498-1-Ig at 1:2000 followed by incubation of HRP-goat anti-mouse secondary antibody. Signal was developed by ECL substrate. A parallel dot blot was performed using Mouse IgG1 isotype control Monoclonal antibody 66360-1-Ig at the same dose.

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Applications testées

• Résultats positifs en ELISA: N4-acetylcytidine
• Résultats positifs en Dot Blot: RNA isolate HeLa cells,

Dilution recommandée

• Test immuno-enzymatique (ELISA): ELISA :
• DOT BLOT: DOT BLOT : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• RIP: See 1 publications below

Informations sur le produit

68498-1-Ig cible N4-acetylcytidine dans les applications de RIP, Dot Blot, ELISA et montre une réactivité avec des échantillons chemical compound

• Réactivité: chemical compound
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Composé
• Nom complet: N4-acetylcytidine
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

N4-Acetylcytidine, CasNo. 3768-18-1, is a modified nucleoside and endogenous urinary nucleoside product of the degradation of tRNA, 18s rRNA and mRNA. N4-Acetylcytidine is a biological marker for various cancers with elevated concentrations present in urine. N4-Acetylcytidine is also a partially protected cytidine and therefore can be used as a synthetic building block to prepare further derivatized nucleosides such as 2',3'-dideoxycytidine. NAT10 catalyzes the formation of N4-acetylcytidine (ac4C) modification on mRNAs, 18S rRNA and tRNAs.

Protocol for Dot Blot:
https://www.ptglab.com/protocol/68498-1-IgDotBlot.pdf

Publications

Species	Application	Title
human	RIP	Int J Biol Macromol; N-acetyltransferase 10 impedes EZH2/H3K27me3/GABARAP axis mediated autophagy and facilitates lung cancer tumorigenesis through enhancing SGK2 mRNA acetylation; Authors - Nan Xiao

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