Anticorps Recombinant de lapin anti-NAC1

NAC1 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

242788E11

N° de cat : 85380-2-RR

Synonymes

NACC1, 242788E11, BEND8, BTB/POZ domain-containing protein 14B, BTBD14B

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Galerie de données de validation

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  WB analysis using 85380-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85380-2-RR (NAC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 85380-2-RR

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using NAC1 antibody (85380-2-RR, Clone: 242788E11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of SH-SY5Y using 85380-2-RR

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using NAC1 antibody (85380-2-RR, Clone: 242788E11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of HeLa using 85380-2-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug NAC1 Recombinant antibody (85380-2-RR, Clone:242788E11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 85380-2-RR

  Biolayer interferometry (BLl) kinetic assays of 85380-2-RR against Human NAC1 were performed. The affinity constant is 1.39 nM.

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules HeLa, cellules K-562, cellules MCF-7, cellules SH-SY5Y
• Résultats positifs en IF/ICC: cellules MCF-7, cellules SH-SY5Y
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85380-2-RR cible NAC1 dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NAC1 Protéine recombinante Ag6096
• Nom complet: nucleus accumbens associated 1, BEN and BTB (POZ) domain containing
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 60-65 kDa
• Numéro d’acquisition GenBank: BC055396
• Symbole du gène: NACC1
• Identification du gène (NCBI): 112939
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NAC1, also named as BTBD14B and NACC1, belongs to the BTB/POZ family. NACC1 is a transcriptional repressor, which is amplified and overexpressed in various human cancers and plays critical roles in tumor development, progression, and drug resistance (PMID: 31101655). While NAC1 has been shown to repress the transcription of two tumor suppressors, Gadd45-g and Gadd45-g- interacting protein 1, scientists have found that NACC1 also plays an important role in nontranscriptional function, for example, it can act as a bridge for MAVS and TBK1 in RLR-mediated signaling (PMID: 31235549). The observed molecular weight is consistent with what has been described in the literature (PMID: 37533751).

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