Anticorps Polyclonal de lapin anti-NADK

• Phare
• Validé par KD/KO

NADK Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 29290-1-PBS

Synonymes

EC:2.7.1.23, NAD kinase, Poly(P)/ATP NAD kinase

---

Galerie de données de validation

• 

  WB analysis using 29290-1-AP (same clone as 29290-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29290-1-AP (NADK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29290-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 29290-1-AP (same clone as 29290-1-PBS)

  WB result of NADK antibody (29290-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-NADK transfected HEK-293 cells. This data was developed using the same antibody clone with 29290-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 29290-1-AP (same clone as 29290-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 29290-1-AP (NADK antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29290-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary cancer using 29290-1-AP (same clone as 29290-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 29290-1-AP (NADK antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29290-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

29290-1-PBS cible NADK dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NADK Protéine recombinante Ag30982
• Nom complet: NAD kinase
• Masse moléculaire calculée: 49 kDa
• Poids moléculaire observé: 49 kDa
• Numéro d’acquisition GenBank: BC001709
• Symbole du gène: NADK
• Identification du gène (NCBI): 65220
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NAD kinase (NADK) is the sole NADP+-biosynthetic enzyme that catalyzes phosphorylation of NAD+ to yield NADP+ using ATP as a phosphoryl donor, and thus, plays a vital role in the cell and represents a potentially powerful antimicrobial drug target (PMID:21526340). It belongs to the NAD kinase family. NADK has 3 isoforms with the molecular mass of 49, 63 and 46 kDa. The catalytically active human NADK is a homotetramer (PMID:11594753).

{{ptg:RelatedPrimaryAntibodies}}