Anticorps Polyclonal de lapin anti-NAGS

NAGS Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(3)

Avis (1)

N° de cat : 21566-1-AP

Synonymes

AGAS, Amino-acid acetyltransferase, ARGA, EC:2.3.1.1, N-acetylglutamate synthase conserved domain form

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Galerie de données de validation

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  WB analysis using 21566-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21566-1-AP (NAGS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 21566-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21566-1-AP (NAGS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 21566-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 21566-1-AP (NAGS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 21566-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21566-1-AP (NAGS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 21566-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21566-1-AP (NAGS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 21566-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21566-1-AP (NAGS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human small intestine using 21566-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 21566-1-AP (NAGS antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human small intestine using 21566-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 21566-1-AP (NAGS antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 21566-1-AP

  Immunofluorescent analysis of HepG2 cells, using NAGS antibody 21566-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  IF Staining of HeLa using 21566-1-AP

  Immunofluorescent analysis of Hela cells, using NAGS antibody 21566-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules HepG2, cellules Jurkat, cellules L02, tissu hépatique de rat
• Résultats positifs en IHC: tissu hépatique humain, tissu d'intestin grêle humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below

Informations sur le produit

21566-1-AP cible NAGS dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NAGS Protéine recombinante Ag16073
• Nom complet: N-acetylglutamate synthase
• Masse moléculaire calculée: 534 aa, 58 kDa
• Poids moléculaire observé: 58-66 kDa
• Numéro d’acquisition GenBank: BC111713
• Symbole du gène: NAGS
• Identification du gène (NCBI): 162417
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

N-acetylglutamate synthase, also known as NAGS, is a mitochondrial enzyme that catalyzes the formation of N-acetylglutamate (NAG) from glutamate and acetyl coenzyme-A. NAGS is the key enzyme for regulating the hepatic urea cycle and is also highly expressed in the kidney and gut. Deficiency of NAGS leads to hyperammonemia due to decreased activity of CPSI deprived of its cofactor NAG(PMID:12459178,12754705).

Publications

Species	Application	Title
mouse	WB	Mol Metab; SWI/SNF complex subunit BAF60a represses hepatic ureagenesis through a crosstalk between YB-1 and PGC-1α.; Authors - Wenxiang Zhang
mouse,human	WB,IHC	Heliyon; Machine learning deciphers the significance of mitochondrial regulators on the diagnosis and subtype classification in non-alcoholic fatty liver disease; Authors - Bingyu Wang
rat	WB	Drug Des Devel Ther; The Potential Mechanisms of Althaea rosea (Linn.) Cavan. Flower in Alleviating Tetrodotoxin Poisoning: An Integrated Metabolomics, Network Pharmacology and Experimental Validation; Authors - Renjin Zheng

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Heaji (Verified Customer) (09-30-2019)

As shown in the image the antibody recognizes a strong band of the wrong size in the NAGS knockout model -- I don't think this works as intended. Thank you.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse liver and intestine

NAGS Antibody Western Blot, validation (1:2000 dilution) in mouse liver and intestine (Cat no:21566-1-AP)

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