Anticorps Polyclonal de lapin anti-NLRP3

• Phare
• Validé par KD/KO

NLRP3 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (3)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA, Cell treatment

Conjugaison

Non conjugué

Publications(594)

Avis (5)

N° de cat : 19771-1-AP

Synonymes

Angiotensin/vasopressin receptor AII/AVP-like, C1orf7, CIAS1, Cold-induced autoinflammatory syndrome 1 protein, EC:3.6.4.-

---

Galerie de données de validation

• 

  WB analysis using 19771-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19771-1-AP (NLRP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 19771-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19771-1-AP (NLRP3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of THP-1 using 19771-1-AP

  IP result of anti-NLRP3 (IP:19771-1-AP, 4ug; Detection:19771-1-AP 1:1000) with THP-1 cells lysate 2480 ug.

• 

  IHC staining of human kidney using 19771-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 19771-1-AP (NLRP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 19771-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 19771-1-AP (NLRP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 19771-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NLRP3 antibody (19771-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 19771-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NLRP3 antibody (19771-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 19771-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NLRP3 antibody (19771-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of THP-1 using 19771-1-AP

  1X10^6 THP-1 cells were intracellularly stained with 0.4 ug Anti-Human NLRP3 (19771-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en WB: cellules THP-1, cellules Raji, cellules THP-1 traitées au LPS
• Résultats positifs en IP: cellules THP-1,
• Résultats positifs en IHC: tissu rénal humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules THP-1

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 19 publications below
• WB: See 507 publications below
• IHC: See 120 publications below
• IF: See 146 publications below
• IP: See 9 publications below
• ELISA: See 1 publications below
• CoIP: See 7 publications below

Informations sur le produit

19771-1-AP cible NLRP3 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA, Cell treatment et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: bovin, Chèvre, Humain, porc
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: NLR family, pyrin domain containing 3
• Masse moléculaire calculée: 118 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: NM_001127461
• Symbole du gène: NLRP3
• Identification du gène (NCBI): 114548
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NALP3, also named C1orf7, CIAS1, and PYPAF1, belongs to the NLRP family. NLRP3, a key and eponymous component of the NLRP3 inflammasome, plays a crucial role in innate immunity and inflammation. NALP3 may function as an inducer of apoptosis. It interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling.NALP3 inhibits TNF-alpha-induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. NALP3 activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18. Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1) which is also known as familial cold urticaria. Defects in NLRP3 are a cause of Muckle-Wells syndrome (MWS) which is urticaria-deafness-amyloidosis syndrome. Defects in NLRP3 are the cause of chronic infantile neurologic cutaneous and articular syndrome (CINCA) which is also known as neonatal onset multisystem inflammatory disease (NOMID). The antibody recognizes the C-term of NALP3.

Publications

Species	Application	Title
	IF	Mol Cell; An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation; Authors - Stephanie Pei Tung Yiu
	WB	J Hazard Mater; Bile acid metabolism is altered in learning and memory impairment induced by chronic lead exposure; Authors - Anfei Liu
human	WB,IF	Sci Adv; Oligodendroglial glycolytic stress triggers inflammasome activation and neuropathology in Alzheimer's disease.; Authors - Xinwen Zhang
	WB	Gut Microbes; Weizmannia coagulans BCF-01: a novel gastrogenic probiotic for Helicobacter pylori infection control; Authors - Zhenhui Chen
	WB	Nat Commun; TRIM28 SUMOylates and stabilizes NLRP3 to facilitate inflammasome activation.; Authors - Ying Qin
	WB	Nat Commun; UAF1 deubiquitinase complexes facilitate NLRP3 inflammasome activation by promoting NLRP3 expression.; Authors - Hui Song

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Jillian (Verified Customer) (05-27-2025)

We tried out three different NLRP3 Rb antibodies on NHP samples and this was the cleanest signal we found. Will be ordering again for future projects.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Liver, Frontal Cortex

FH

Avelina (Verified Customer) (04-14-2025)

We titrated this antibody for use in NHP in kidney and liver/gallbladder and staining appears nonspecific. Will be repeating testing at lower concentrations to determine specificity.

• Applications: Immunohistochemistry, Immunofluorescence
• Cell Tissue Type: NHP Kidney & Liver

FH

Anita (Verified Customer) (09-20-2024)

Initially had trouble getting the WB to work. Reached out to technical support. Had not realised that Proteintech actually develops protocols that are specific for the antibody. Followed the tech support recommendations as well as WB protocol and the WB worked out better than expected.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: rat placenta

FH

Macarena Lucia (Verified Customer) (10-17-2022)

clear band.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

FH

Tanusree (Verified Customer) (12-03-2019)

This antibody works good in western blotting analysis using mouse tissues.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain

{{ptg:RelatedPrimaryAntibodies}}