Anticorps Monoclonal anti-NAMPT/PBEF

• Phare
• Validé par KD/KO

NAMPT/PBEF Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

CloneNo.

3D4D8

N° de cat : 66385-1-Ig

Synonymes

1110035O14Rik, NAmPRTase, NAMPT, NAMPT/PBEF, PBEF, PBEF1, Pre B cell enhancing factor, VF, VISFATIN

---

Galerie de données de validation

• 

  WB analysis using 66385-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66385-1-Ig (NAMPT/PBEF antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis using 66385-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66385-1-Ig (NAMPT/PBEF antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis of human heart using 66385-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 66385-1-Ig (NAMPT/PBEF antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 66385-1-Ig

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66385-1-Ig (NAMPT/PBEF antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 66385-1-Ig

  rat heart tissue were subjected to SDS PAGE followed by western blot with 66385-1-Ig (NAMPT/PBEF antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 66385-1-Ig

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66385-1-Ig (NAMPT/PBEF antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 66385-1-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66385-1-Ig (NAMPT/PBEF antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 66385-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66385-1-Ig (NAMPT/PBEF antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human breast cancer using 66385-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66385-1-Ig (NAMPT/PBEF antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 66385-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66385-1-Ig(NAMPT/PBEF antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules HCT 116, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules HT-29, cellules Jurkat, cellules K-562, cellules MCF-7, cellules MOLT-4, cellules Neuro-2a, cellules NIH/3T3, cellules PC-12, cellules PC-3, tissu cardiaque de rat, tissu cardiaque humain, tissu de muscle squelettique de souris, tissu de muscle squelettique humain
• Résultats positifs en IHC: tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Informations sur le produit

66385-1-Ig cible NAMPT/PBEF dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NAMPT/PBEF Protéine recombinante Ag2434
• Nom complet: nicotinamide phosphoribosyltransferase
• Masse moléculaire calculée: 52 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC020691
• Symbole du gène: NAMPT
• Identification du gène (NCBI): 10135
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Nicotinamide phosphoribosyltransferase(NAMPT) has two usual synonyms termed Visfatin and PBEF. Its primary role is to catalyze the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD, which is the rate limiting component in the mammalian NAD biosynthesis pathway. NAMPT is localized in cytoplasm and expressed in large amounts in bone marrow, liver tissue, and muscle tissues. NAMPT inhibits neutrophil apoptosis in experimental inflammation and clinical sepsis. NAMPT levels are altered in plasma of patients with type 2 diabetes mellitus (T2DM), and it is now evidenced that NAMPT may plays a role in lipid metabolism.

Publications

Species	Application	Title
human	WB,IHC	J Cell Physiol; Nicotinamide phosphoribosyl transferase regulates cell growth via the Sirt1/P53 signaling pathway and is a prognosis marker in colorectal cancer.; Authors - Jing-Hua Pan ; KD Validated
human	WB	Cell Rep; NAD+ supplement potentiates tumor-killing function by rescuing defective TUB-mediated NAMPT transcription in tumor-infiltrated T cells.; Authors - Yuetong Wang
human	WB	Cell Chem Biol; Addressing the Enzyme-independent tumor-promoting function of NAMPT via PROTAC-mediated degradation; Authors - Xiaotong Zhu
mouse	WB	Int J Mol Sci; Regulation of NAD+/NADH Redox Involves the Protective Effects of Ginsenoside Rb1 against Oxygen-Glucose Deprivation/Reoxygenation-Induced Astrocyte Lesions; Authors - Ying Liu